RP-HPLC analysis of methanol extract of Viscum articulatum
| Autor: | Joydeb Chanda, Pulok K. Mukherjee, Amit Kar, Mrinmoy Nag |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Viscum articulatum Burm
Analytical Techniques and Drug Discovery Method validation 0211 other engineering and technologies 02 engineering and technology Loranthaceae 03 medical and health sciences Acetic acid chemistry.chemical_compound 0302 clinical medicine Betulinic acid 021105 building & construction Drug Discovery Oleanolic acid Lupeol Betulin Chromatography biology Antimicrobial biology.organism_classification 030205 complementary & alternative medicine Complementary and alternative medicine chemistry Original Research Article (Experimental) RP-HPLC Methanol Ayurveda |
| Zdroj: | Journal of Ayurveda and Integrative Medicine |
| ISSN: | 0976-2809 0975-9476 |
| Popis: | Background Viscum articulatum Burm. (Family: Loranthaceae) is commonly known as mistletoe. In ayurveda, the plant parts are used in “Kapha”, “Vata”, diseases of the blood, ulcer, and epilepsy. The plant parts are also used in urinary tract infection and wound infection. The plant contains five triterpenoids such as α-amyrin, lupeol, betulin, betulinic acid and oleanolic acid, exhibiting several pharmacological activities including antimicrobial, anti-HIV, antitumor, antiviral activity. Objective To ensure the content of uniformity of oleanolic acid, a RP-HPLC method has been developed for estimation of oleanolic acid in V. articulatum aerial part. Material and methods The RP-HPLC method was carried out in reverse phase C18 column, using methanol and water as mobile phase in the ratio of 95:5 (v/v), at the flow rate of 1 mL/min. The pH of aqueous phase was adjusted 3.2 with 1% (v/v) glacial acetic acid. The λmax was set at 210 nm. Results The retention time of oleanolic acid was found at 21.5 ± 0.05 min. The linearity of the response was found to be 10–800 μg/mL. The coefficient of determinants of oleanolic acid was found to be (r2) 0.995 and equation Y = 19462X + 16,172. The LOD and LOQ were found to be for oleanolic acid (1.96% w/w) 0.197 ± 0.63 and 0.623 ± 0.87 μg/mL, respectively. The developed method was accurate, specific, precise and reproducible. Conclusion This RP-HPLC may be useful for quantitative estimation of the chemical constituents present in the plant extract as well as the quality assessment of the herbal product. Highlights • An ethnomedicinal plant – Viscum articulatum used for analysis. • Method validation of oleanolic acid through RP-HPLC. • Quality control of Ayurvedic medicinal Plant. |
| Databáze: | OpenAIRE |
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