Mapping biological to clinical phenotypes during the development (21 days) and resolution (21 days) of experimental gingivitis
| Autor: | Ann E. Scott, Fionnuala Lundy, Gerard J. Linden, Iain L. C. Chapple, Monica J. Carlile, John B. Matthews, Mojgan A. Naeeni, Michael R. Milward, S. Lorraine Martin, Brian Walker, Denis F. Kinane, Gareth R. Brock |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Cathepsin G Adolescent Interleukin-1beta Paired difference test Dental Plaque Physiology Substance P Young Adult chemistry.chemical_compound Gingivitis Reference Values medicine Humans Single-Blind Method Volunteer Inflammation biology business.industry Elastase Gingival Crevicular Fluid Middle Aged Oral Hygiene Phenotype chemistry Neutrophil elastase biology.protein Periodontics Female medicine.symptom Leukocyte Elastase business Biomarkers |
| Zdroj: | Journal of Clinical Periodontology. 39:123-131 |
| ISSN: | 0303-6979 |
| DOI: | 10.1111/j.1600-051x.2011.01825.x |
| Popis: | Aim: To characterize and map temporal changes in the biological and clinical phenotype during a 21-day experimental gingivitis study. Materials and Methods: Experimental gingivitis was induced over 21 days in healthy human volunteers (n = 56), after which normal brushing was resumed (resolution phase). Gingival and plaque indices were assessed. Gingival crevicular fluid was collected from four paired test and contra-lateral control sites in each volunteer during induction (Days 0, 7, 14 and 21) and resolution (Days 28 and 42) of experimental gingivitis. Fluid volumes were measured and a single analyte was quantified from each site-specific, 30s sample. Data were evaluated by analysis of repeated measurements and paired sample tests. Results: Clinical indices and gingival crevicular fluid volumes at test sites increased from Day 0, peaking at Day 21 (test/control differences all p < 0.0001) and decreased back to control levels by Day 28. Levels of four inflammatory markers showed similar patterns, with significant differences between test and control apparent at Day 7 (substance P, cathepsin G, interleukin-1b, elastase: all p < 0.03) and peaking at Day 21 (all p < 0.002). Levels of a-1-antitrypsin showed no pattern. Conclusions: Levels of substance P, cathepsin G, interleukin-1b and neutrophil elastase act as objective biomarkers of gingival inflammation induction and resolution that typically precede phenotypical changes. |
| Databáze: | OpenAIRE |
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