Drosophila imaginal disc growth factor 2 is a trophic factor involved in energy balance, detoxification, and innate immunity
| Autor: | Lenka Rouhova, Vaclav Broz, Lucie Kucerova, Hynek Strnad, Jana Fleischmannova, Michal Zurovec, Peter J. Bryant |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Biology Article 03 medical and health sciences Immunity Hemolymph medicine Extracellular Animals Drosophila Proteins Cells Cultured Glycoproteins Multidisciplinary Innate immune system Ecology Gene Expression Profiling Cytoprotection Adenosine In vitro Immunity Innate Cell biology Gene expression profiling Imaginal disc 030104 developmental biology Inactivation Metabolic Drosophila Energy Metabolism medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep43273 |
| Popis: | Drosophila imaginal disc growth factor 2 (IDGF2) is a member of chitinase-like protein family (CLPs) able to induce the proliferation of imaginal disc cells in vitro. In this study we characterized physiological concentrations and expression of IDGF2 in vivo as well as its impact on the viability and transcriptional profile of Drosophila cells in vitro. We show that IDGF2 is independent of insulin and protects cells from death caused by serum deprivation, toxicity of xenobiotics or high concentrations of extracellular adenosine (Ado) and deoxyadenosine (dAdo). Transcriptional profiling suggested that such cytoprotection is connected with the induction of genes involved in energy metabolism, detoxification and innate immunity. We also show that IDGF2 is an abundant haemolymph component, which is further induced by injury in larval stages. The highest IDGF2 accumulation was found at garland and pericardial nephrocytes supporting its role in organismal defence and detoxification. Our findings provide evidence that IDGF2 is an important trophic factor promoting cellular and organismal survival. |
| Databáze: | OpenAIRE |
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