32P distribution into 2,3-bisphosphoglyceric acid from human erythrocytes following molybdate-catalyzed hydrolysis
| Autor: | L. Michael Snyder, Richard E. Rice, Lena L. Neri, Normand Fortier |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Erythrocytes
Time Factors Biophysics Molybdate Biochemistry Catalysis Hydrolysis chemistry.chemical_compound Methods Humans Phosphoric Acids Molecular Biology Incubation Molybdenum 2 3-Bisphosphoglyceric acid Chromatography Phosphotransferases Phosphate Diphosphoglyceric Acids Phosphorus-32 chemistry Isotope Labeling Specific activity Phosphorus Radioisotopes |
| Zdroj: | Biochimica et biophysica acta. 381(1) |
| ISSN: | 0006-3002 |
| Popis: | Normal human washed erythrocytes were incubated in autologous plasma with 3 2P for varying periods of time. 2,3-Bisphosphoglyceric acid the major organic phosphate ester of red cells, was first isolated and purified by anion-exchange chromatography; subsequently, the C-2 Pi was selectively hydrolyzed by a molybdate-catalyzed reaction. The C-2-bound Pi of 2,3-P2-glyceric acid was found to have a significantly higher specific activity than C-3 Pi. Intramolecular equilibration was not reached until the 180-min incubation. No evidence of overhydrolysis or internal randomization by the molybdate reaction was noted which was confirmed following hydrolysis of double-label 2,3-P2-glyceric acid3 2P C-2, 3 3P C-3. |
| Databáze: | OpenAIRE |
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