Low Molecular Weight Heparin Relieves Experimental Colitis in Mice by Downregulating IL-1β and Inhibiting Syndecan-1 Shedding in the Intestinal Mucosa

Autor: Youlian Zhou, Xia Wang, Cun-long Chen, Shi-yong Lin, Ye Chen, Side Liu, Ai-ming Li, Chudi Chen, Jing Li, Xianfei Wang
Rok vydání: 2013
Předmět:
Male
Anatomy and Physiology
Mouse
Interleukin-1beta
Digestive Physiology
lcsh:Medicine
Pharmacology
Mice
Intestinal mucosa
Intestinal Mucosa
lcsh:Science
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Dextran Sulfate
Interleukin
Animal Models
Heparin
Colitis
Immunohistochemistry
Ulcerative colitis
Interleukin-10
Medicine
medicine.symptom
Research Article
medicine.drug
Cell Physiology
Drugs and Devices
Drug Research and Development
Immunoblotting
Immunology
Down-Regulation
Inflammation
Gastroenterology and Hepatology
Proinflammatory cytokine
Model Organisms
medicine
Animals
Ulcerative Colitis
Biology
Acute colitis
DNA Primers
Analysis of Variance
business.industry
lcsh:R
Inflammatory Bowel Disease
Immunity
Heparin
Low-Molecular-Weight
medicine.disease
Mice
Inbred C57BL
carbohydrates (lipids)
lcsh:Q
Clinical Immunology
Syndecan-1
business
Digestive System
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 7, p e66397 (2013)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0066397
Popis: Low molecular weight heparin (LMWH) exhibits anti-inflammatory properties, but its effect on inflammation in colitis remains unclear. This study aimed to evaluate the therapeutic effects of LMWH on dextran sulfate sodium (DSS)-induced colitis in mice, in which acute colitis progresses to chronic colitis, and to explore the potential mechanism involved in this process. C57BL/6 mice were randomly divided into control, DSS, and DSS plus LMWH groups (n = 18). Disease activity was scored by a disease activity index (DAI). Histological changes were evaluated by hematoxylin and eosin (HE) staining. The mRNA levels of syndecan-1, interleukin (IL)-1β, and IL-10 were determined by quantitative reverse transcription polymerase chain reaction. Protein expression of syndecan-1 was detected by immunohistochemistry. The serum syndecan-1 level was examined by a dot immunobinding assay. LMWH ameliorated the disease activity of colitis induced by DSS administration in mice. Colon destruction with the appearance of crypt damage, goblet cell loss, and a larger ulcer was found on day 12 after DSS administration, which was greatly relieved by the treatment of LMWH. LMWH upregulated syndecan-1 expression in the intestinal mucosa and reduced the serum syndecan-1 level on days 12 and 20 after DSS administration (P
Databáze: OpenAIRE
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