Curauá-derived carbon dots: Fluorescent probes for effective Fe(III) ion detection, cellular labeling and bioimaging
| Autor: | Luiz H. C. Mattoso, Sebastian Raja, Eva Miriam Buhl, Martin Zenke, Antonio Sechi, Carmen Schalla, Stephan Dreschers |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Ananas erectifolius Iron Bioengineering Ferric Compounds Flow cytometry Biomaterials Mice Live cell imaging Quantum Dots medicine Animals Fluorescent Dyes Detection limit Aqueous solution medicine.diagnostic_test biology biology.organism_classification Fluorescence Carbon Spectrometry Fluorescence Linear range Mechanics of Materials Ferric Nuclear chemistry medicine.drug |
| Zdroj: | Materials Science and Engineering: C. 129:112409 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2021.112409 |
| Popis: | This study reports the generation of curaua-derived carbon dots (C-dots) and their suitability for Fe(III) detection, bioimaging and FACS analysis. C-dots were generated from curaua (Ananas erectifolius) fibers by a facile one-step hydrothermal approach. They exhibited graphite-like structure with a mean diameter of 2.4 nm, high water solubility, high levels of carboxyl and hydroxyl functional groups, excitation-dependent multicolor fluorescence emission (in the range 450 nm - 560 nm) and superior photostability. C-dots were highly selective and effective for the detection of ferric Fe(III) ion in an aqueous medium with a detection limit of 0.77 μM in the linear range of 0–30 μM, a value much lower than the guideline limits proposed by the World Health Organization (WHO). In biological cell systems, C-dots were very well tolerated by B16F1 mouse melanoma and J774.A1 mouse macrophages cell lines, both of which effectively internalized C-dots in their cytoplasmic compartment. Finally, C-dots were effective probes for long-term live cell imaging experiments and multi-channel flow cytometry analysis. Collectively, our findings demonstrate that curaua-derived C-dots serve as versatile and effective natural products for Fe(III) ion sensing, labeling and bioimaging of various cell types. This study adds novel C-dots to the library of carbon-based probes and paves the way towards a sustainable conversion of a most abundant biomass waste into value-added products. |
| Databáze: | OpenAIRE |
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