Histopathological and biochemical evaluation of the effect of Paeoniflorin on the periodontium during and after periodontitis formation in rats
| Autor: | Şevki Güler, Gonca Çayır Keleş Tezal, Alper Çiftci, Pınar Naile Gürgör, Burcu Ozkan Cetinkaya, Sevda Kurt, Çağla Gül Gürkan |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty animal structures Histology medicine.medical_treatment Alveolar Bone Loss Gastroenterology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glucosides stomatognathic system Internal medicine medicine Animals Rats Wistar Periodontitis Ligature General Dentistry Saline Dental alveolus Experimental periodontitis business.industry 030206 dentistry Cell Biology General Medicine Periodontium medicine.disease Paeoniflorin Rats stomatognathic diseases 030104 developmental biology Otorhinolaryngology chemistry Clinical attachment loss Monoterpenes Cytokines Matrix metalloproteinase(s) business |
| ISSN: | 0004-7120 |
| Popis: | WOS: 000471206900018 PubMed: 31005686 Objectives: Appraise the effect of systemic Paeoniflorin (Pae) application on the periodontium during and after induction of experimental periodontitis in the presence of ligature and after its removal. Design: Seventy male Wistar rats were separated into seven equal groups. The first group was reserved as healthy control group (Group 1: no periodontitis, no medication) and experimental periodontitis was induced with ligature in the remaining rats. In experimental periodontitis groups, Pae or saline was administered systemically in three differet periods; during the experimental periodontitis induction (period 1), after experimental periodontitis induction which ligature removed (period 2) or ligature kept in position (period 3). Only, one of the groups acted as the control periodontitis group and received no treatment. Experimental periodontitis groups were as follows; Group 2: medication in period 1, Group 3: periodontitis and no medication, Group 4: medication in period 2, Group 5: saline application in period 2, Group 6: medication in period 3, Group 7: saline application in period 3. Matrix metalloproteinases-9 (MMP-9) levels and interleukin-10 (IL-10) levels were detected biochemically and histomorphometric analyses were performed. These analyses included measurements of the area of alveolar bone, the level of alveolar bone, and attachment loss. Results: Area of alveolar bone and IL-10 levels were higher in the Pae-administered groups; level of alveolar bone, attachment loss, and MMP-9 levels were correspondingly lower (P < 0.05). The beneficial effects at histomorphometrical and biochemical levels of Pae were the strongest in the rats that were administered Pae after the removal of ligature. Conclusions: Systemically administered Pae had a positive effect on the healing of periodontal tissues. Pae can be used as a new therapeutic agent for periodontal diseases, but microbiology-based studies and more extensive biochemistry-based experimental and clinical studies are needed to address this possibility. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115S846] This study was supported by the The Scientific and Technological Research Council of Turkey (TUBITAK) (Project number: 115S846). |
| Databáze: | OpenAIRE |
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