A Novel ALL-L3 Cell Line, BALM-25, Expressing Both Immunoglobulin Light Chains
| Autor: | Kunzo Orita, Yoshinobu Matsuo, Katsuto Takenaka, Toshimichi Ishihara, Hans G. Drexler, Fumihiko Ishimaru, Akira Harashima |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Epstein-Barr Virus Infections Cancer Research Blotting Western Biology Immunoglobulin light chain CD19 Immunophenotyping Antigens CD Cell Line Tumor hemic and lymphatic diseases medicine Humans Southern blot Chromosome Aberrations Gene Rearrangement Hematology Middle Aged medicine.disease Burkitt Lymphoma Molecular biology Isotype Blotting Southern Leukemia Oncology biology.protein Immunoglobulin Light Chains CD5 Antibody |
| Zdroj: | Leukemia & Lymphoma. 45:575-581 |
| ISSN: | 1029-2403 1042-8194 |
| Popis: | A human acute lymphoblastic leukemia (ALL)-derived cell line, BALM-25, was established from the bone marrow specimen of a 59-year-old male patient with B-cell ALL L3 type (ALL-L3) at diagnosis. Immunophenotyping indicated mature B-cell characteristics including expression of cell surface and cytoplasmic immunoglobulin (Ig) chains, CD10, CD19, CD20, CD38, CD39, CD40, CD71, NU-B1 and HLA class II. T-cell and myeloid associated antigens tested were negative except CD5. BALM-25 cells have a morphological appearance typical for L3-type lymphoblasts. Regarding the expression of Ig chains, while the original leukemia cells expressed Ig lambda delta mu and hence a single light (L) chain isotype, the established line revealed double L chain expression both at the cell surface and the cytoplasmic level. Definitive double L chain expression was confirmed by flow cytometry and Western blot analysis. Southern blot analysis demonstrated rearrangement of the IgJH, the Ckappa and the Clambda genes. Cytogenetic analysis of BALM-25 revealed the following numerical and structural abnormalities: 55, X, add(X)(q12), + 2, add(3)(p21), + 5, add(7)(p13), add(11)(p11.2), add(11)(q?23), add(12)(p11.2), add(14)(q22), - 15, + 16, + 16,add(18)(11.2), + 20, + marl, + mar2, + mar3, + mar,inc. The established cell line, BALM-25, provides an unlimited supply of cell material for analyzing the unique (patho)physiology of Ig expression in general and for clarifying the pathogenesis of this type of B-cell malignancy in particular. |
| Databáze: | OpenAIRE |
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