The DNA co-vaccination using Sm antigen and IL-10 as prophylactic experimental therapy ameliorates nephritis in a model of lupus induced by pristane

Autor: Oscar Pizano-Martínez, Flavio Sandoval-García, Mónica Vázquez-Del Mercado, Erika Aurora Martínez-García, Minoru Satoh, Rogelio Hernández-Pando, Fernanda Isadora Corona-Meraz, Trinidad García-Iglesias, Beatriz Teresita Martín-Márquez, Marcelo H. Petri
Rok vydání: 2021
Předmět:
Physiology
Lupus nephritis
DNA vaccination
Autoantigens
Biochemistry
Epitope
Mice
Immune Physiology
Vaccines
DNA
Medicine and Health Sciences
Lupus Erythematosus
Systemic
Public and Occupational Health
Enzyme-Linked Immunoassays
Mice
Inbred BALB C
Innate Immune System
Multidisciplinary
Systemic lupus erythematosus
Immune System Proteins
medicine.diagnostic_test
biology
Therapies
Investigational
Vaccination
Animal Models
Interleukin-10
Precipitation Techniques
Experimental Organism Systems
Medicine
Vaccination and immunization
Cytokines
Female
Antibody
Research Article
Science
Immunology
Mouse Models
Immunofluorescence
Research and Analysis Methods
Systemic Lupus Erythematosus
Antibodies
Autoimmune Diseases
Model Organisms
Antigen
Rheumatology
medicine
Animals
Immunoprecipitation
Immunoassays
Autoantibodies
Lupus Erythematosus
business.industry
Prophylaxis
Autoantibody
Biology and Life Sciences
Proteins
Molecular Development
medicine.disease
Immune System
biology.protein
Immunologic Techniques
Animal Studies
Clinical Immunology
Preventive Medicine
Clinical Medicine
business
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 10, p e0259114 (2021)
PLoS ONE, Vol 16, Iss 10 (2021)
ISSN: 1932-6203
Popis: Introduction Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies such as anti-Sm. Studies in patients with SLE and murine models of lupus reveal that the most critical anti-Sm autoantibodies are predominantly direct against D1(83–119), D2, and B´/B epitopes. Objectives The present study aimed to analyze the induction of antigen-specific tolerance after prophylactic immunization with a DNA vaccine encoding the epitopes: D183-119, D2, B´/B, and B´/BCOOH in co-vaccination with IFN-γ or IL-10 in a murine model of lupus induced by pristane. Material and methods To obtain endotoxin-free DNA vaccines, direct cloning techniques using pcDNA were performed: D183-119, D2, B´/B, B´/BCOOH, IFN-γ, or IL-10. Lupus was induced by 0.5 mL of pristane via intraperitoneal in BALB/c female mice. Immunoprecipitation with K562 cells was metabolically labeled with 35S and ELISA to detect serum antibodies or mice IgG1, IgG2a isotypes. ELISA determined IL-10 and IFN-γ from splenocytes supernatants. Proteinuria was assessed monthly, and lupus nephritis was evaluated by immunofluorescence, and electron microscopy. Results The prophylactic co-vaccination with D2/IL-10 reduced the expression of kidney damage observed by electron microscopy, direct immunofluorescence, and H & E, along with reduced level of anti-nRNP/Sm antibodies (P = 0.048). Conclusion The prophylactic co-vaccination of IL-10 with D2 in pristane-induced lupus ameliorates the renal damage maybe by acting as prophylactic DNA tolerizing therapy.
Databáze: OpenAIRE
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