Protein Tyrosine Phosphatase Receptor Type R (PTPRR) Reduces AChR Clustering by Dephosphorylating MuSK

Autor:	Yanxun Chen, Maohao Guan, Fengqiang Yu, Zhongshan Yang, Weiqiang Yi, Xuan Huang, Ruiqin Qiu, Fancai Lai
Rok vydání:	2022
Předmět:	Article Subject Biochemistry (medical) Clinical Biochemistry Genetics Cluster Analysis Humans Receptor Protein-Tyrosine Kinases Receptors Cholinergic General Medicine Receptor-Like Protein Tyrosine Phosphatases Class 7 Protein Tyrosine Phosphatases Molecular Biology Acetylcholine
Zdroj:	Disease markers. 2022
ISSN:	1875-8630
Popis:	Neuromuscular junction (NMJ) formation and maintenance depend on the proper localization and concentration of various molecules at synaptic contact sites. Acetylcholine receptor (AChR) clustering on the postsynaptic membrane is a cardinal event in NMJ formation. Muscle-specific tyrosine kinase (MuSK), which functions depending on its phosphorylation, plays an essential role in AChR clustering. In the present study, we used plasmid-based biochemical screening and determined that protein tyrosine phosphatase receptor type R (PTPRR) is responsible for dephosphorylating MuSK on tyrosine residue 754. Furthermore, we showed that PTPRR significantly reduced MuSK-dependent AChR clustering in C2C12 myotubes. Collectively, these data illustrate a negative regulation function of PTPRR in AChR clustering.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd83b9796f65b1a2f7c781fdc346772f https://pubmed.ncbi.nlm.nih.gov/36105254 Zobrazit plný text záznamu Plný text