Determination of sodium artesunate in plasma using ion-pairing high-performance liquid chromatography
| Autor: | R.G. Reid, R.R. Moody, M.I Awad, R.B. Taylor |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Detection limit
Chromatography Cationic polymerization Artesunate Reproducibility of Results General Chemistry Reference Standards High-performance liquid chromatography Sensitivity and Specificity Artemisinins Ion chemistry.chemical_compound Antimalarials chemistry Bromide Reference Values Calibration Humans Spectrophotometry Ultraviolet Acetonitrile Quantitative analysis (chemistry) Sesquiterpenes Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 744(2) |
| ISSN: | 1387-2273 |
| Popis: | A chromatographic method is described for the determination of sodium artesunate in plasma. This includes cetyltrimethylammonium bromide as a cationic pairing ion in a reversed-phase system using an octadecylsilica 100×4.6 mm I.D. 3 μm analytical column with a mobile phase of acetonitrile/acetate buffer at pH7. Column switching incorporating a 5 μm octadecylsilica 100×4.6 mm I.D. precolumn is used in addition to off-line solid-phase extraction for pretreatment of plasma samples in order to eliminate interference from endogenous components. Detection is by post-column derivatisation with 1.0 M methanolic KOH followed by UV detection at 289 nm. Calibration is linear over the range 100–1600 ng ml −1 and the limit of detection is estimated as 20 ng ml −1 . Illustrative results are shown of the artesunate plasma levels determined by the proposed method following the administration of artesunate as tablets and as suppositories to healthy volunteers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect