PID1 (NYGGF4), a new growth-inhibitory gene in embryonal brain tumors and gliomas
Autor: | Anat, Erdreich-Epstein, Nathan, Robison, Xiuhai, Ren, Hong, Zhou, Jingying, Xu, Tom B, Davidson, Mathew, Schur, Floyd H, Gilles, Lingyun, Ji, Jemily, Malvar, Gregory M, Shackleford, Ashley S, Margol, Mark D, Krieger, Alexander R, Judkins, David T W, Jones, Stefan M, Pfister, Marcel, Kool, Richard, Sposto, Shahab, Asgharzadeh, Shahab, Asgharazadeh |
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Rok vydání: | 2013 |
Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Gene Expression Kaplan-Meier Estimate Biology Article Disease-Free Survival Glioma Cell Line Tumor medicine Humans RNA Messenger Cerebellar Neoplasms neoplasms Protein kinase B Proportional Hazards Models Medulloblastoma Mesenchymal stem cell Wnt signaling pathway Cancer Neoplasms Germ Cell and Embryonal medicine.disease nervous system diseases Oncology Apoptosis Child Preschool Atypical teratoid rhabdoid tumor Female Carrier Proteins |
Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 20(4) |
ISSN: | 1557-3265 |
Popis: | Purpose: We present here the first report of PID1 (Phosphotyrosine Interaction Domain containing 1; NYGGF4) in cancer. PID1 was identified in 2006 as a gene that modulates insulin signaling and mitochondrial function in adipocytes and muscle cells. Experimental Design and Results: Using four independent medulloblastoma datasets, we show that mean PID1 mRNA levels were lower in unfavorable medulloblastomas (groups 3 and 4, and anaplastic histology) compared with favorable medulloblastomas (SHH and WNT groups, and desmoplastic/nodular histology) and with fetal cerebellum. In two large independent glioma datasets, PID1 mRNA was lower in glioblastomas (GBM), the most malignant gliomas, compared with other astrocytomas, oligodendrogliomas and nontumor brains. Neural and proneural GBM subtypes had higher PID1 mRNA compared with classical and mesenchymal GBM. Importantly, overall survival and radiation-free progression-free survival were longer in medulloblastoma patients whose tumors had higher PID1 mRNA (univariate and multivariate analyses). Higher PID1 mRNA also correlated with longer overall survival in patients with glioma and GBM. In cell culture, overexpression of PID1 inhibited colony formation in medulloblastoma, atypical teratoid rhabdoid tumor (ATRT), and GBM cell lines. Increasing PID1 also increased cell death and apoptosis, inhibited proliferation, induced mitochondrial depolaization, and decreased serum-mediated phosphorylation of AKT and ERK in medulloblastoma, ATRT, and/or GBM cell lines, whereas siRNA to PID1 diminished mitochondrial depolarization. Conclusions: These data are the first to link PID1 to cancer and suggest that PID1 may have a tumor inhibitory function in these pediatric and adult brain tumors. Clin Cancer Res; 20(4); 827–36. ©2013 AACR. |
Databáze: | OpenAIRE |
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