In vivo antimalarial evaluation of Gomphostenins
| Autor: | A.K. Srivastava, Mahabir Parshad Kaushik, Manisha Sathe, Ramarao Ghorpade |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
food.ingredient
Plasmodium berghei medicine.medical_treatment Drug Evaluation Preclinical Biology Pharmacology Pharmacognosy Antimalarials Mice food In vivo Chloroquine Drug Discovery medicine Animals Saline Chromatography High Pressure Liquid Lamiaceae Dose-Response Relationship Drug Plant Extracts Gomphostemma medicine.disease biology.organism_classification Terpenoid Malaria medicine.drug |
| Zdroj: | Journal of Ethnopharmacology. 130:171-174 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2010.04.006 |
| Popis: | Aim of the study The present study aims to evaluate the in vivo antimalarial potential of the leaf extract of Gomphostemma niveum and two new compounds; named as Gomphostenin (GN–6) and acetyl Gomphostenin (GN–10) isolated and purified from this plant against Plasmodium berghei in mice. Materials and methods The blood schizontocidal activity was performed in early infection of Plasmodium berghei and also in established infection of Plasmodium berghei . The mice were orally administrated with various doses of water and chloroform extracts of leaves of Gomphostemma niveum (GN–W and GN–C, 300, 400 and 500 mg/kg/day), GN–6 and GN–10 (50, 100, 150 and 200 mg/kg/day). Chloroquine (8 mg/kg) used as a positive control, while an equal volume of saline plus 0.5% tween-80 was used as a negative control. Results GN–W, GN–C and pure compounds (GN–6 and GN–10) produced a dose dependent chemosuppression effect at various dose levels. GN–10 exhibited highest percent of chemosuppression i.e. 92.65% at a dose level of 200 mg/kg/day. In case of curative test, the survival period of the parasitized infected mice was significantly prolonged at 200 mg/kg dose of GN–10. Conclusions The studies have indicated that clerodane class of diterpenes GN–6 and GN–10 certainly holds great promise for malaria control and will be useful in antimalarial chemotherapy. |
| Databáze: | OpenAIRE |
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