Characterization of the dinophysistoxin-2 acute oral toxicity in mice to define the Toxicity Equivalency Factor
| Autor: | José Manuel Cifuentes, Mercedes R. Vieytes, Natalia Vilariño, Amparo Alfonso, M. Carmen Louzao, Inés Rodríguez, Paula Abal, Luis M. Botana |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Administration Oral Urine Pharmacology Biology Toxicology Median lethal dose Lethal Dose 50 Eating Feces Mice 03 medical and health sciences Tandem Mass Spectrometry Okadaic Acid Toxicity Tests medicine Animals Ingestion Pyrans Gastrointestinal tract Body Weight General Medicine Acute toxicity Intestines Diarrhea 030104 developmental biology Liver Toxicity Female Marine Toxins medicine.symptom Chromatography Liquid Food Science |
| Zdroj: | Food and Chemical Toxicology. 102:166-175 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2017.02.023 |
| Popis: | Ingestion of shellfish with dinophysistoxin-2 (DTX2) can lead to diarrheic shellfish poisoning (DSP). The official control method of DSP toxins in seafood is the liquid chromatography-mass spectrometry analysis (LC-MS). However in order to calculate the total toxicity of shellfish, the concentration of each compound must be multiplied by individual Toxicity Equivalency Factor (TEF). Considering that TEFs caused some controversy and the scarce information about DTX2 toxicity, the aim of this study was to characterize the oral toxicity of DTX2 in mice. A 4-Level Up and Down Procedure allowed the characterization of DTX2 effects and the estimation of DTX2 oral TEF based on determination of the lethal dose 50 (LD50). DTX2 passed the gastrointestinal barrier and was detected in urine and feces. Acute toxicity symptoms include diarrhea and motionless, however anatomopathology study and ultrastructural images restricted the toxin effects to the gastrointestinal tract. Nevertheless enterocytes microvilli and tight junctions were not altered, disconnecting DTX2 diarrheic effects from paracellular epithelial permeability. This is the first report of DTX2 oral LD50 (2262 μg/kg BW) indicating that its TEF is about 0.4. This result suggests reevaluation of the present TEFs for the DSP toxins to better determine the actual risk to seafood consumers. |
| Databáze: | OpenAIRE |
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