In vitro effect of recombinant human gonadotropins on meiotic competence of dog oocytes
| Autor: | Selin Yağcioğlu, Mithat Evecen, Nur Ersoy, Serhat Pabuccuoğlu, Kemal Ak, Ramazan Arici, Hatem Atalla, Nilhan Coşkun, Sema Birler, Kamber Demir |
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| Přispěvatelé: | İÜC, Veteriner Fakültesi, Klinik Bilimler Bölümü, Coşkun, Nilhan, Demir, Kamber, Evecen, Mithat, Arıcı, Ramazan, Yağcıoğlu, Selin, Ersoy, Nur, Atalla, Hatem, Ak, Kemal, Birler, Sema, Pabuccuoglu, Serhat, Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM) |
| Jazyk: | angličtina |
| Předmět: |
Veterinary sciences
endocrine system General Veterinary Significant difference Dog oocyte in vitro maturation recombinant gonadotropin hormone Biology Dog oocyte in vitro maturation In vitro In vitro maturation law.invention Andrology Recombinant gonadotropin hormone Meiosis law Reproductive biology Recombinant DNA recombinant gonadotropin hormone Gonadotropins pituitary Hormone |
| Zdroj: | Volume: 43, Issue: 4 469-473 Turkish Journal of Veterinary and Animal Sciences |
| ISSN: | 1300-0128 1303-6181 |
| Popis: | The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulus-oocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium was supplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH and rhLH) gonadotropins. After the IVM period, the maturation rate of the oocytes was investigated under an epifluorescence microscope. Our findings showed no significant difference in maturation rate using either pituitary or human recombinant gonadotropin groups (P > 0.05). Applying 1.0 IU human recombinant gonadotropin caused the lowest maturation rate (34.57%; P < 0.05). In conclusion, recombinant human gonadotropins could be applied for IVM of dog oocytes. Moreover, 0.05 IU/mL rhFSH and rhLH can be successfully used in place of biologically derived hormones. Scientific Research Projects Coordination Unit of Istanbul University |
| Databáze: | OpenAIRE |
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