Adherence of Aeromonas hydrophila strains to human enterocyte-like cells pre-infected with Rotavirus

Autor:	M P, Bertuccio, I, Picerno, M E, Scoglio
Rok vydání:	2012
Předmět:	Rotavirus Enterocytes Coinfection Humans Caco-2 Cells Bacterial Adhesion Aeromonas hydrophila
Popis:	Introduction. The interest grown in these years about emerging pathogens in the onset of intestinal disease showed that the patho- genic mechanism is a multifactorial event. Our objective was to evaluate the role of co-infection with rotavirus in the expression of Aeromonas spp adhesiveness. Methods. The rate of co-infection involves contact of Caco-2 cells with the virus, followed by adsorption for 1 and 2 hours. Aliquots of bacterial suspensions were added to tissue-culture plates. After infection, cell monolayers were lysed; serially diluted lysates were plated to determine the number of bound bacteria by performing colony forming units (CFU) counts. Results. Non-adhesive strains were not subject to variations resulting from co-infection, while those who had medium or high adhesiveness gave rise to an increase of the same. Discussion. Infection with rotavirus promotes the Aeromonas ability to adhere to Caco-2 cells and this effect depends on the duration of infection and on the starting adhesiveness of bacteria strain. Journal of Preventive Medicine and Hygiene, Vol 53, No 3 (2012)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd77b3e0de464673512bf1ce88a378d0 http://hdl.handle.net/11570/2433425 Zobrazit plný text záznamu