Genetic and epigenetic coordination of cortical interneuron development
| Autor: | Mariano I. Gabitto, Giuseppe A. Saldi, Rachel C. Bandler, Gord Fishell, Richard Bonneau, Orly Wapinski, Chen Yu Wang, Kathryn C. Allaway, Sherry Jingjing Wu |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Candidate gene Interneuron Neurogenesis Gene regulatory network Article Epigenesis Genetic Mice Cell Movement Interneurons Cortex (anatomy) medicine Animals Gene Regulatory Networks Epigenetics RNA-Seq Cerebral Cortex Mice Knockout Multidisciplinary biology MEF2 Transcription Factors Cell Differentiation Chromatin medicine.anatomical_structure Parvalbumins Cerebral cortex biology.protein Female Single-Cell Analysis Somatostatin Neuroscience Parvalbumin |
| Zdroj: | Nature |
| ISSN: | 1476-4687 |
| Popis: | One of the hallmarks of the cerebral cortex is the extreme diversity of interneurons1–3. The two largest subtypes of cortical interneurons, parvalbumin- and somatostatin-positive cells, are morphologically and functionally distinct in adulthood but arise from common lineages within the medial ganglionic eminence4–11. This makes them an attractive model for studying the generation of cell diversity. Here we examine how developmental changes in transcription and chromatin structure enable these cells to acquire distinct identities in the mouse cortex. Generic interneuron features are first detected upon cell cycle exit through the opening of chromatin at distal elements. By constructing cell-type-specific gene regulatory networks, we observed that parvalbumin- and somatostatin-positive cells initiate distinct programs upon settling within the cortex. We used these networks to model the differential transcriptional requirement of a shared regulator, Mef2c, and confirmed the accuracy of our predictions through experimental loss-of-function experiments. We therefore reveal how a common molecular program diverges to enable these neuronal subtypes to acquire highly specialized properties by adulthood. Our methods provide a framework for examining the emergence of cellular diversity, as well as for quantifying and predicting the effect of candidate genes on cell-type-specific development. The transcriptional programs and chromatin accessibility changes that cause two related interneuronal subtypes to diverge from common developmental origins and acquire specialized properties are elucidated. |
| Databáze: | OpenAIRE |
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