Calcium signaling mediates a biphasic mechanoadaptive response of endothelial cells to cyclic mechanical stretch
| Autor: | Eva Faurobert, Xinping Li, Yekaterina A. Miroshnikova, Corinne Albiges-Rizo, Sara A. Wickström, Sandra Manet |
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| Přispěvatelé: | Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Max planck Institute for Biology of Ageing [Cologne], Helsingin yliopisto = Helsingfors universitet = University of Helsinki, FRM, ANR-17-CE13-0022,CODECIDE,Coincidence de detection dans l'identité cellulaire(2017), ALBIGES-RIZO, Corinne, Coincidence de detection dans l'identité cellulaire - - CODECIDE2017 - ANR-17-CE13-0022 - AAPG2017 - VALID, University of Helsinki |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cytochalasin D
Filamins Alpha catenin [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Mechanotransduction Cellular Ion Channels Antigens CD Human Umbilical Vein Endothelial Cells [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Humans Calcium Signaling Protein Interaction Maps Mechanotransduction Molecular Biology Actin Calcimycin Calcium signaling biology Cell Biology Actomyosin Adherens Junctions Articles Vinculin Cadherins Phosphoproteins Biomechanical Phenomena rac GTP-Binding Proteins Actin Cytoskeleton Calcium Ionophores p21-Activated Kinases biology.protein Biophysics rhoA GTP-Binding Protein |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, 2021, 32 (18), pp.1724-1736. ⟨10.1091/mbc.E21-03-0106⟩ Mol Biol Cell Molecular Biology of the Cell, American Society for Cell Biology, 2021, 32 (18), pp.1724-1736. ⟨10.1091/mbc.E21-03-0106⟩ |
| ISSN: | 1939-4586 |
| Popis: | International audience; The vascular system is precisely regulated to adjust blood flow to organismal demand, thereby guaranteeing adequate perfusion under varying physiological conditions. Mechanical forces, such as cyclic circumferential stretch, are among the critical stimuli that dynamically adjust vessel distribution and diameter, but the precise mechanisms of adaptation to changing forces are unclear. We find that endothelial monolayers respond to cyclic stretch by transient remodeling of the vascular endothelial cadherin-based adherens junctions and the associated actomyosin cytoskeleton. Time-resolved proteomic profiling reveals that this remodeling is driven by calcium influx through the mechanosensitive Piezo1 channel, triggering Rho activation to increase actomyosin contraction. As the mechanical stimulus persists, calcium signaling is attenuated through transient down-regulation of Piezo1 protein. At the same time, filamins are phosphorylated to increase monolayer stiffness, allowing mechanoadaptation to restore junctional integrity despite continuing exposure to stretch. Collectively, this study identifies a biphasic response to cyclic stretch, consisting of an initial calcium-driven junctional mechanoresponse, followed by mechanoadaptation facilitated by monolayer stiffening. |
| Databáze: | OpenAIRE |
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