Photocurrent Generation of Reconstituted Photosystem II on a Self-Assembled Gold Film
| Autor: | Daiki Nishiori, Hiroshi Nishihara, Masako Iwai, Mariko Miyachi, Yoshinori Yamanoi, Shu Ikehira, Masato Yamada, Suleyman I. Allakhverdiev, Tatsuya Tomo |
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| Rok vydání: | 2017 |
| Předmět: |
Photocurrent
Absorption spectroscopy Photosystem II Chemistry Analytical chemistry 02 engineering and technology Surfaces and Interfaces 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Photochemistry Platinum nanoparticles 01 natural sciences 0104 chemical sciences Molecular wire Electrode Electrochemistry General Materials Science Irradiation 0210 nano-technology Spectroscopy Deposition (law) |
| Zdroj: | Langmuir. 33:1351-1358 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/acs.langmuir.6b03499 |
| Popis: | Photosystem II (PSII)-modified gold electrodes were prepared by the deposition of PSII reconstituted with platinum nanoparticles (PtNPs) on Au electrodes. PtNPs modified with 1-[15-(3,5,6-trimethyl-1,4-benzoquinone-2-yl)]pentadecyl disulfide ((TMQ(CH2)15S)2) were incorporated into the QB site of PSII isolated from thermophilic cyanobacterium Thermosynechococcus elongatus. The reconstitution was confirmed by QA-reoxidation measurements. PSII reconstituted with PtNPs was deposited and integrated on a Au(111) surface modified with 4,4′-biphenyldithiol. The cross section of the reconstituted PSII film on the Au electrode was investigated by SEM. Absorption spectra showed that the surface coverage of the electrode was about 18 pmol PSII cm–2. A photocurrent density of 15 nAcm–2 at E = +0.10 V (vs Ag/AgCl) was observed under 680 nm irradiation. The photoresponse showed good reversibility under alternating light and dark conditions. Clear photoresponses were not observed in the absence of PSII and molecular wire... |
| Databáze: | OpenAIRE |
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