Glycerol Enhancement of Ligand-Polylysine/DNA Transfection
| Autor: | Walter Schmidt, Antoine Kichler, W. Zauner, Ernst Wagner, A. Sinski |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Glycerol
Ethylene Glycol Biology Ligands Transfection Endocytosis General Biochemistry Genetics and Molecular Biology Cell Line chemistry.chemical_compound Genes Reporter Gene expression Cell Adhesion medicine Animals Humans Polylysine Enzyme Inhibitors Promoter Regions Genetic Fibroblast Reporter gene Chloroquine DNA Fibroblasts beta-Galactosidase Ligand (biochemistry) Molecular biology Anti-Bacterial Agents Clone Cells medicine.anatomical_structure Gene Expression Regulation Biochemistry chemistry Cell culture Interleukin-2 Ethylene Glycols Macrolides Plasmids Biotechnology |
| Zdroj: | BioTechniques. 20:905-913 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/96205rr04 |
| Popis: | Primary human fibroblasts and a series of cell lines (A549, BNL CL.2, H225, NIH 3T3 and Rat-1) are efficiently transfected by using positively charged complexes of plasmid DNA and transferrin-polylysine or polylysine in the presence of glycerol (1 molar to 1.8 molar, depending on the cell type). An increase in gene expression of up to several- hundredfold (compared to complexes without glycerol) is obtained if the transfection mixture is incubated with the cells for 3–4 h at 37°C. This simple method has been used for transient expression of luciferase, b-galactosidase and interleukin-2, and also for the generation of stably transfected cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|