Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma
| Autor: | Yu-Long Wang, Jia-Qian Hu, Qinghai Ji, Yu Wang, Xiaohua Hu, Xiaoming Zhang, Zhongwu Lu, Wen-Jun Wei, Ning Qu, Huajun Li, Hualei Gan, Li-Cheng Tan, Peng-Cheng Yu, Weilin Pu, Zaili Luo, Zhiyan Liu, Jiucun Wang, Dongmei Ji, Xiao Shi, Meiying Zhang, Pei-Zhen Han |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Adolescent endocrine system diseases Tumour heterogeneity medicine.medical_treatment Science Population Cell General Physics and Astronomy Biology Article Thyroid cancer General Biochemistry Genetics and Molecular Biology Iodine Radioisotopes Thyroid carcinoma Transcriptome Cancer genomics medicine Humans Thyroid Neoplasms education Ecosystem education.field_of_study Multidisciplinary Carcinoma RNA sequencing General Chemistry Immunotherapy Phenotype Gene Expression Regulation Neoplastic Developmental trajectory medicine.anatomical_structure Thyroid Cancer Papillary Thyroid Epithelial Cells Cancer research Lymph Nodes Single-Cell Analysis Radioactive iodine |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | The tumor ecosystem of papillary thyroid carcinoma (PTC) is poorly characterized. Using single-cell RNA sequencing, we profile transcriptomes of 158,577 cells from 11 patients’ paratumors, localized/advanced tumors, initially-treated/recurrent lymph nodes and radioactive iodine (RAI)-refractory distant metastases, covering comprehensive clinical courses of PTC. Our data identifies a “cancer-primed” premalignant thyrocyte population with normal morphology but altered transcriptomes. Along the developmental trajectory, we also discover three phenotypes of malignant thyrocytes (follicular-like, partial-epithelial-mesenchymal-transition-like, dedifferentiation-like), whose composition shapes bulk molecular subtypes, tumor characteristics and RAI responses. Furthermore, we uncover a distinct BRAF-like-B subtype with predominant dedifferentiation-like thyrocytes, enriched cancer-associated fibroblasts, worse prognosis and promising prospect of immunotherapy. Moreover, potential vascular-immune crosstalk in PTC provides theoretical basis for combined anti-angiogenic and immunotherapy. Together, our findings provide insight into the PTC ecosystem that suggests potential prognostic and therapeutic implications. The characterisation of the papillary thyroid carcinoma (PTC) tumour microenvironment remains crucial. Here, the authors perform single-cell RNA sequencing in 11 patients and identify potential opportunities for the use of immunotherapy and its combination with anti-angiogenic therapy in PTC. |
| Databáze: | OpenAIRE |
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