Isolation of antibacterial response genes from the dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) immunized with Escherichia coli

Autor:	Eun-Young Yun, Iksoo Kim, Kim Young-Tae, Hea-Son Bang, Jae-Sam Hwang, Kim Seong-Ryul, Kim Yeon-Ju, Kwan-Ho Park, Seok-Jo Hwang, Jae-Pil Jeon, Kim Nam-Soon
Rok vydání:	2008
Předmět:	Scarabaeidae biology dung beetle hsp70 Dot blot antibacterial gene biology.organism_classification medicine.disease_cause Hsp70 Microbiology QL1-991 Insect Science Heat shock protein Complementary DNA Botany medicine Zoology Escherichia coli Gene Dung beetle
Zdroj:	European Journal of Entomology, Vol 105, Iss 2, Pp 355-359 (2008)
ISSN:	1802-8829 1210-5759
DOI:	10.14411/eje.2008.042
Popis:	Dot blot hybridization of total RNA from normal and bacteria-injected larvae of the dung beetle Copris tripartitus identi- fied 13 cDNA clones that seem to be associated with the immune response. A cDNA encoding a dung beetle homolog of the heat shock protein HSP70 (CTHSP70) was characterized by full-length sequencing and expression pattern analysis. CTHSP70 was upregulated 4 h after bacterial injection, reached maximum level after 8 h, and declined gradually after 16 h. Our data suggest that CTHSP70 and another 12 genes are involved in the anti-microbial defense that is particularly important for dung beetle larvae living in pathogen-rich conditions.
Databáze:	OpenAIRE
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