Temporal biological variability in dendritic cells and regulatory T cells in peripheral blood of healthy adults
| Autor: | Maleewan Kitcharoensakkul, Huiqing Yin-Declue, Jonathan S. Boomer, Dana Burgdorf, Brad Wilson, Kenneth B. Schechtman, Mario Castro, Leonard B. Bacharier |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Time Factors Myeloid medicine.medical_treatment CD3 Immunology T-Lymphocytes Regulatory Peripheral blood mononuclear cell Article 03 medical and health sciences 0302 clinical medicine medicine Humans Immunology and Allergy biology FOXP3 Dendritic Cells Healthy Volunteers In vitro Peripheral blood Blood Cell Count Surgery 030104 developmental biology medicine.anatomical_structure Cytokine Granzyme Health 030220 oncology & carcinogenesis Leukocytes Mononuclear biology.protein Cytokines Female |
| Zdroj: | Journal of Immunological Methods. 431:63-65 |
| ISSN: | 0022-1759 |
| Popis: | Studies evaluating circulating dendritic cells (DCs) and natural and induced regulatory T cells (nTregs, iTregs) are often obtained at a single time point and difficult to interpret without understanding their intrinsic day-to-day biologic variability.We investigated the day-to-day variability in quantifying DCs, nTregs (FoxP3(+)CD25(+)CD4(+)) and cytokine production by iTregs (granzyme B-GZB, Th1/2 cytokines following CD3 plus CD46 in vitro activation) from peripheral blood mononuclear cells (PBMCs) collected on three consecutive days in healthy adults. Intraclass correlation coefficients (ICCs) were used to evaluate intra-individual variability.In 10 healthy adults, the %PBMCs of plasmacytoid (pDC) and myeloid (mDC1 and mDC2) were 0.27 ± 0.12, 0.22 ± 0.10, and 0.02 ± 0.02, with ICC 0.91, 0.90, and 0.17 respectively. Natural Tregs (3.27 ± 1.27% CD4(+) cells) had an ICC of 0.86. Inducible Tregs (GZB-positive, 35.3 ± 17.7% CD4(+) cells) had an ICC of 0.77. The ICCs for IL-10, TNF-α, IFN-γ, IL-4, and IL-5 production by iTregs were 0.49, 0.63, 0.68, 0.74, and 0.82, respectively. There were no significant changes in ICC (0.1) after adjusting for age, gender and atopy except for IL-4. Substantial variability for iTregs was determined for the control condition (PBS with IL-2).No meaningful day-to-day biologic variability was observed for the quantification of nTregs, pDC and mDC1 in normal adults; however, there was substantial variability in measuring mDC2 proportions and iTreg production of IL-10. These results suggest obtaining an average of several measurements over time to determine the most representative value of these biologic measures. |
| Databáze: | OpenAIRE |
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