High Titer Anti-Basement Membrane Antibodies in a Subset of Patients with Pediatric Systemic Lupus Erythematosus
| Autor: | Debra Canter, Adisak Suwanichkul, Alvaro Orjuela, Scott E. Wenderfer, M. John Hicks, Sridevi Devaraj, Eyal Muscal, Charles G. Minard |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Mice Inbred MRL lpr Pathology medicine.medical_specialty Adolescent Lupus nephritis Enzyme-Linked Immunosorbent Assay Article Basement Membrane Immunoglobulin G Mice medicine Animals Humans Lupus Erythematosus Systemic Child Autoantibodies Lupus erythematosus Systemic lupus erythematosus biology business.industry Autoantibody Glomerulonephritis DNA medicine.disease Lupus Nephritis Antibodies Anti-Idiotypic Immunoglobulin A Mice Inbred C57BL Disease Models Animal Immunoglobulin M Nephrology Antibodies Antinuclear Case-Control Studies Immunology biology.protein Female business Nephritis Biomarkers |
| Zdroj: | American Journal of Nephrology. 41:241-247 |
| ISSN: | 1421-9670 0250-8095 |
| Popis: | Background/Aims: There is a critical need for more noninvasive biomarkers to identify nephritis in patients with systemic lupus erythematosus (SLE). Recent studies in a model mouse and an adult SLE patient cohort suggest that anti-basement membrane antibody levels correlate well with lupus activity and kidney injury. The purpose of this study was to assess the anti-basement membrane reactivity in pediatric SLE (pSLE) patients with or without nephritis. Methods: Auto-antibodies to basement membrane antigens were assessed using an anti-matrigel ELISA. Endpoint titers were measured in pSLE patients and healthy children, as well as in autoimmune and non-immune mice, with good reproducing capabilities. Findings were also analyzed with respect to the presence or absence of nephritis, dsDNA antibodies, and other manifestations of pSLE. Results: MRL/lpr mice developed high-titer anti-matrigel antibodies, whereas C57BL/6 mice did not. In a cohort of 21 pSLE patients and 22 pediatric controls, high-titer anti-matrigel IgG, IgM and IgA antibody levels were specific for pSLE. High-titer anti-matrigel IgG3 levels could distinguish with good sensitivity the 13 pSLE patients with a history of nephritis from the 8 non-renal pSLE patients. High-titer anti-matrigel IgG, IgA, IgM or IgG3 did not correlate with positive anti-double stranded DNA, but defined an overlapping subset of patients. Conclusion: The addition of anti-basement membrane antibody testing to serologic testing in pSLE may help to monitor disease activity or to define important subsets of patients with risks for specific disease manifestations. |
| Databáze: | OpenAIRE |
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