ZBTB gene expression in HIV patients: a possible new molecular mechanism of viral control
| Autor: | Santiago Ávila-Ríos, Mariana del Rocio Ruiz-Briseño, Sarah Ratkovich-Gonzalez, Monserrat Alvarez-Zavala, Moisés Ramos-Solano, José Luis Mosqueda-Gómez, Karina Sánchez-Reyes, Judith Carolina De Arcos-Jiménez, Luz A. González-Hernández, Jaime Andrade-Villanueva |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Repressor Gene Expression HIV Infections Biology Virus Replication Cell Line 03 medical and health sciences Downregulation and upregulation Virology Gene expression Transcriptional regulation Gene family Gene silencing Humans Gene Transcription factor 030304 developmental biology 0303 health sciences 030306 microbiology General Medicine Cell biology Virus Latency DNA-Binding Proteins Gene Expression Regulation HIV-1 Leukocytes Mononuclear Female Transcription Factors |
| Zdroj: | Archives of virology. 166(1) |
| ISSN: | 1432-8798 |
| Popis: | HIV infects its target cell and integrates into its genome as an essential step in its replication cycle. Proviral DNA is also subjected to the same transcriptional regulation as the host cell genome by its own transcriptional factors, with activating or repressive activity. There is a clear interaction between the presence of transcriptional repressors and a decrease in the rate of HIV replication, promoting gene silencing in infected cells, which serve as viral reservoirs. This represents a major obstacle for HIV eradication. The ZBTB gene family comprises 49 genes that encode transcription factors that have a repressor function in differentiation and development of cells of the lymphopoietic lineage, including the main target cells of HIV, CD4+ T cells. In this cross-sectional study, we evaluated the expression profile of ZBTB genes in CD4+ T cells of HIV-positive individuals with different levels of infection control. We found upregulation of gene expression of ZBTB4 (p < 0.01), ZBTB7B (p < 0.001), and ZBTB38 (p < 0.05) and downregulation of ZBTB16 (p < 0.01) in HIV-positive patients compared to HIV-negative individuals. Interestingly, in a deeper analysis, we observed that elite controllers had the highest levels of expression of the ZBTB38, ZBTB2, HIC1, ZBTB7A, ZBTB7B (ThPOK) and ZBTB4 genes, showing 2.56- to 7.60-fold upregulation compare to the ART-naive group. These results suggest a possible contribution of these ZBTB transcriptional repressors in HIV-positive patients and a possible new molecular mechanism of viral control. |
| Databáze: | OpenAIRE |
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