Ferritin-induced relaxation in tissues: An in vitro study

Autor: Alain Roch, Yves Gossuin, Robert N. Muller, Gérard Toubeau, Anne Monseux, Carmen Burtea, Pierre Gillis
Rok vydání: 2004
Předmět:
Zdroj: Journal of Magnetic Resonance Imaging. 20:690-696
ISSN: 1522-2586
1053-1807
DOI: 10.1002/jmri.20152
Popis: 1Purpose: To study in vitro the proton relaxation induced intissues by ferritin, the iron-storing protein of mammals.Materials and Methods: Nuclear magnetic relaxation dis-persion (NMRD) profiles of liver and spleen from control andiron-overloaded mice are compared with NMRD profiles offerritin and Fercayl —a ferritin-like akaganeite particle—inaqueous solutions or in 1% agarose gel.Results: The relaxation of water protons induced by ferritinand Fercayl in 1% agarose gel is comparable with therelaxation of aqueous solutions of the same compounds,but slower than the relaxation of liver and spleen. The gel isnot a good model of tissues containing ferritin. The longi-tudinal NMRD profiles of control and iron-overloaded liverand spleen are almost identical: ferritin accumulation hasonly a slight effect on longitudinal relaxation. The trans-verse NMRD profiles of liver and spleen tissues are linear,but the slope of the linear regression is larger for iron-loaded organs than for control ones, which is a conse-quence of a higher ferritin concentration in the former.However, the correlation between the slope of the trans-verse NMRD profiles and the iron concentration is not verygood, probably because transverse relaxation is modifiedby the clustering of ferritin in cells.Conclusion: It could be difficult to develop a general tech-nique for the accurate quantification of ferritin-bound iron bynuclear magnetic resonance or magnetic resonance imaging.Key Words: ferritin; relaxation; iron content; tissue; NMRDprofileJ. Magn. Reson. Imaging 2004;20:690–696.© 2004 Wiley-Liss, Inc.
Databáze: OpenAIRE
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