Aquaporins influence seed dormancy and germination in response to stress
| Autor: | Steven Footitt, Rachel Clewes, Lorenzo Frigerio, William E. Finch-Savage, Mistianne Feeney |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
dormancy Physiology Water flow Arabidopsis Aquaporin Germination Plant Science Vacuole Biology Environment global warming Aquaporins 01 natural sciences tonoplast intrinsic protein abscisic acid 03 medical and health sciences chemistry.chemical_compound water stress Plant Growth Regulators Stress Physiological Botany Abscisic acid 030304 developmental biology 0303 health sciences Arabidopsis Proteins QK Seed dormancy Temperature Gene Expression Regulation Developmental Membrane Proteins Water Original Articles Plant Dormancy Plants Genetically Modified QP aquaporin chemistry Seeds Dormancy Original Article Cycling 010606 plant biology & botany |
| Zdroj: | Plant, Cell & Environment |
| ISSN: | 1365-3040 0140-7791 |
| Popis: | Aquaporins influence water flow in plants, yet little is known of their involvement in the water‐driven process of seed germination. We therefore investigated their role in seeds in the laboratory and under field and global warming conditions. We mapped the expression of tonoplast intrinsic proteins (TIPs) during dormancy cycling and during germination under normal and water stress conditions. We found that the two key tonoplast aquaporins, TIP3;1 and TIP3;2, which have previously been implicated in water or solute transport, respectively, act antagonistically to modulate the response to abscisic acid, with TIP3;1 being a positive and TIP3;2 a negative regulator. A third isoform, TIP4;1, which is normally expressed upon completion of germination, was found to play an earlier role during water stress. Seed TIPs also contribute to the regulation of depth of primary dormancy and differences in the induction of secondary dormancy during dormancy cycling. Protein and gene expression during annual cycling under field conditions and a global warming scenario further illustrate this role. We propose that the different responses of the seed TIP contribute to mechanisms that influence dormancy status and the timing of germination under variable soil conditions. Aquaporins influence water flow in plants, yet little is known of their involvement in the water‐driven process of seed germination. We therefore investigated their role in seeds, both in the laboratory and field. We mapped the expression of tonoplast intrinsic proteins during germination in normal and water stress conditions. We found that the two key tonoplast aquaporins, TIP3;1 and TIP3;2, act antagonistically to modulate the response to ABA, with TIP3;1 being a positive and TIP3;2 a negative regulator. A third isoform, TIP4;1, which is normally expressed upon completion of germination, was found to play an earlier role during water stress. Seed TIPs also regulate the depth of primary dormancy and differences in the induction of secondary dormancy in a similar manner. We propose that the different responses of the seed TIP constitute a mechanism to influence germination under water stress and dormancy cycling in variable soil conditions. |
| Databáze: | OpenAIRE |
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