Antibody response to toxic shock syndrome toxin-1 of Staphylococcus aureus in dairy cows
| Autor: | Takeshi Shimano, Hirofumi Komae, Osamu Funaki, Shotaro Takeuchi, Yuji Hayakawa, Michiko Hayashi, Toshio Kaidoh, Mamoru Akagi |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Staphylococcus aureus Micrococcaceae Somatic cell Bacterial Toxins Cattle Diseases Cell Count Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Microbiology Polymerase Chain Reaction Serology Enterotoxins medicine Animals Lactation DNA Primers Electrophoresis Agar Gel Superantigens General Veterinary Toxic shock syndrome General Medicine Staphylococcal Infections biology.organism_classification medicine.disease Antibodies Bacterial Shock Septic Mastitis Milk biology.protein Cattle Female Antibody Somatic cell count |
| Zdroj: | Veterinary microbiology. 72(3-4) |
| ISSN: | 0378-1135 |
| Popis: | Antibody response to toxic shock syndrome toxin-1 (TSST-1) of Staphylococcus aureus in dairy cows was examined by enzyme linked immunosorbent assay (ELISA). Serum antibody to TSST-1 was not detected in 39 (76.5%) of 51 calves, which were 1–6 months of age. In contrast, TSST-1 antibody was demonstrated in 1728 (72.6%) of 2380 lactating cows housed on 36 dairy farms. The ELISA values of antibody ranged from 0.2 to 3.0 OD and presented a distribution with the peak at 1.6 OD. The mean ELISA value differed between farms, and it increased slightly along with parturient history. Somatic cell counts of milk from 174 lactating cows was compared with TSST-1 antibody and tst gene examined by polymerase chain reaction (PCR). The mean ELISA values in sera were approximately the same value (1.143–1.297 OD) in cows, with somatic cell counts in milk ranging from 1,000,000 cells per ml. The mean ELISA values in milk were lower than those of sera, but they rose as somatic cells increased. The tst gene of S. aureus was detected in 76.0–86.2% of the milk samples containing somatic cells >500,000 cells per ml, a level which indicates mastitis. The data suggests that many lactating cows may be infected by TSST-1- producing S. aureus . |
| Databáze: | OpenAIRE |
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