Coordination among tertiary base pairs results in an efficient frameshift-stimulating RNA pseudoknot
| Autor: | Kung-Yao Chang, Hao-Teng Hu, Kai-Chun Chang, Cheng-Fu Chang, Yu-Ting Chen, Jin-Der Wen, Yi-Lan Chen, You-Hsin Lin, Chiung-Fang Hsu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular Reading Frames Optical Tweezers Base pair viruses Computational biology Biology Ribosome Frameshift mutation Substrate Specificity 03 medical and health sciences Genetics Protein biosynthesis Fluorescence Resonance Energy Transfer RNA Messenger Base Pairing Nuclear Magnetic Resonance Biomolecular Messenger RNA Oligoribonucleotides 030102 biochemistry & molecular biology Frameshifting Ribosomal Mechanical resistance Rna pseudoknot 030104 developmental biology RNA Nucleic Acid Conformation Pseudoknot Ribosomes |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Frameshifting is an essential process that regulates protein synthesis in many viruses. The ribosome may slip backward when encountering a frameshift motif on the messenger RNA, which usually contains a pseudoknot structure involving tertiary base pair interactions. Due to the lack of detailed molecular explanations, previous studies investigating which features of the pseudoknot are important to stimulate frameshifting have presented diverse conclusions. Here we constructed a bimolecular pseudoknot to dissect the interior tertiary base pairs and used single-molecule approaches to assess the structure targeted by ribosomes. We found that the first ribosome target stem was resistant to unwinding when the neighboring loop was confined along the stem; such constrained conformation was dependent on the presence of consecutive adenosines in this loop. Mutations that disrupted the distal base triples abolished all remaining tertiary base pairs. Changes in frameshifting efficiency correlated with the stem unwinding resistance. Our results demonstrate that various tertiary base pairs are coordinated inside a highly efficient frameshift-stimulating RNA pseudoknot and suggest a mechanism by which mechanical resistance of the pseudoknot may persistently act on translocating ribosomes. |
| Databáze: | OpenAIRE |
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