Photodynamic diagnostics for stomach cancer detection in stress modeled lesions in vivo
| Autor: | Nikita A. Navolokin, Ekaterina Borisova, Alexander Khorovodov, Matvey Kanevsky, Oxana Semyachkina-Glushkovskaya, Anastasia A. Shnitenkova, Svetlana A. Konnova, Veronika E. Shimanova, Tatiana S. Vashenko, Anna A. Galitskaya, Aysel Mamedova, Ilana Agranovich |
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| Rok vydání: | 2019 |
| Předmět: |
POL
Gastrointestinal tract Pathology medicine.medical_specialty stomach cancer Protoporphyrin IX business.industry Atrophic gastritis Stomach Cancer photosensitizers medicine.disease 5-ALA/PpIX adenocarcenoma chemistry.chemical_compound medicine.anatomical_structure chemistry photodynamic diagnostics medicine Photosensitizer Protoporphyrin business Stomach cancer |
| Zdroj: | Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine. |
| DOI: | 10.1117/12.2520148 |
| Popis: | Gastric cancer is the main cause of death among oncological diseases of the gastrointestinal tract, which is associated with the difficulty of early diagnosis of this disease. This study aims to develop a new method for diagnosing gastric cancer using the delta-aminolevulinic acid / protoporphyrin IX photosensitizer (5-ALA / PpIX). The experiments were performed on white rats of average weight 250 grams. The animals were divided into 2 groups: control and experimental (stress + diet, including nitrites and carcinogenic amines) ones. The results of the studies, averaged for each type of tissue, clearly show significant differences between normal, precancerous and malignant changes in the stomach tissues of rats on the background of application of photodynamic diagnostics. The fluorescence from malignant neoplasms in the organ was maximal at 635 nm, which corresponds to the maximum emission of protoporphyrin due to the high accumulation of this photosensitizer in the affected tissues. The most important result of the study was the detection of precancerous changes (atrophic gastritis, confirmed by histological methods), using photodynamic diagnostics with photosensitizer 5-ALA / PpIX. Intensity of fluorescence in case of precancerous changes occupied an intermediate value between that in normal and in malignant mucosa of the stomach. |
| Databáze: | OpenAIRE |
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