ZmFAR1 and ZmABCG26 Regulated by microRNA Are Essential for Lipid Metabolism in Maize Anther

Autor:	Ziwen Li, An Xueli, Xiangyuan Wan, Xinze Liu, Quancan Hou, Mengbing Yang, Ke Xie, Shaowei Zhang, Jing Wang, Rongrong Xie, Tingwei Yan, Canfang Niu, Jinping Li, Yilin Jiang, Taotao Zhu
Rok vydání:	2021
Předmět:	0106 biological sciences 0301 basic medicine QH301-705.5 Mutant Mutagenesis (molecular biology technique) Biology 01 natural sciences Article noncoding RNA Catalysis Inorganic Chemistry Transcriptome 03 medical and health sciences Lipid biosynthesis lipid metabolism anther and pollen development Biology (General) Physical and Theoretical Chemistry maize (Zea mays) QD1-999 Molecular Biology Gene Spectroscopy microRNA Organic Chemistry Wild type Lipid metabolism General Medicine Phenotype Computer Science Applications Chemistry 030104 developmental biology Biochemistry genic male sterility 010606 plant biology & botany
Zdroj:	International Journal of Molecular Sciences Volume 22 Issue 15 International Journal of Molecular Sciences, Vol 22, Iss 7916, p 7916 (2021)
ISSN:	1422-0067
DOI:	10.3390/ijms22157916
Popis:	The function and regulation of lipid metabolic genes are essential for plant male reproduction. However, expression regulation of lipid metabolic genic male sterility (GMS) genes by noncoding RNAs is largely unclear. Here, we systematically predicted the microRNA regulators of 34 maize white brown complex members in ATP-binding cassette transporter G subfamily (WBC/ABCG) genes using transcriptome analysis. Results indicate that the ZmABCG26 transcript was predicted to be targeted by zma-miR164h-5p, and their expression levels were negatively correlated in maize B73 and Oh43 genetic backgrounds based on both transcriptome data and qRT-PCR experiments. CRISPR/Cas9-induced gene mutagenesis was performed on ZmABCG26 and another lipid metabolic gene, ZmFAR1. DNA sequencing, phenotypic, and cytological observations demonstrated that both ZmABCG26 and ZmFAR1 are GMS genes in maize. Notably, ZmABCG26 proteins are localized in the endoplasmic reticulum (ER), chloroplast/plastid, and plasma membrane. Furthermore, ZmFAR1 shows catalytic activities to three CoA substrates in vitro with the activity order of C12:0-CoA > C16:0-CoA > C18:0-CoA, and its four key amino acid sites were critical to its catalytic activities. Lipidomics analysis revealed decreased cutin amounts and increased wax contents in anthers of both zmabcg26 and zmfar1 GMS mutants. A more detailed analysis exhibited differential changes in 54 monomer contents between wild type and mutants, as well as between zmabcg26 and zmfar1. These findings will promote a deeper understanding of miRNA-regulated lipid metabolic genes and the functional diversity of lipid metabolic genes, contributing to lipid biosynthesis in maize anthers. Additionally, cosegregating molecular markers for ZmABCG26 and ZmFAR1 were developed to facilitate the breeding of male sterile lines.
Databáze:	OpenAIRE
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