Differential Expression of Acid Invertase Genes during Seed Germination inArabidopsis thaliana
| Autor: | Wataru Mitsuhashi, Young-Yell Yang, Shigekazu Sasaki, Yuji Kamiya, Tomonobu Toyomasu, Akihiko Kanazawa |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Arabidopsis Germination Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Gene Expression Regulation Plant Gene expression Botany Arabidopsis thaliana RNA Messenger Molecular Biology beta-Fructofuranosidase biology Phytochrome Reverse Transcriptase Polymerase Chain Reaction Organic Chemistry General Medicine Triazoles Blotting Northern biology.organism_classification Gibberellins Invertase Seedlings Seedling Seeds Gibberellin Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 68:602-608 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.68.602 |
| Popis: | In Arabidopsis thaliana (L.) Heynh ecotype Landsberg, levels of soluble acid invertase activity are closely related to the progress of seed germination. To study the mechanism(s) of the development of these enzymes, two cDNA clones that encode putative vacuolar acid invertases were isolated from germinating seeds and very young seedlings using reverse-transcription polymerase chain reactions with degenerate primers. These fragments corresponded to the genes At beta fruct3 and At beta fruct4 from the Columbia ecotype. An apoplasmic invertase gene corresponding to At beta fruct1/ATCWINV1 was also isolated from these samples. Northern blot analyses showed that At beta fruct3 and At beta fruc4 are expressed concomitantly with germination and the subsequent seedling growth. In contrast, the At beta fruct1/AtcwINV1 mRNA is translated before germination. These expression patterns are regulated by phytochrome, which perceives red light and in turn triggers de novo synthesis of gibberellin, initiating Arabidopsis seed germination. To test the effects of gibberellin on the expression of these genes, seed were treated with a gibberellin biosynthesis inhibitor, uniconazole or prohexadione. These chemicals inhibited both seed germination and expression of the above genes, but subsequently applied GA(4), an active gibberellin, reversed the inhibition. These results suggest that the transcription of genes encoding the vacuolar invertases, At beta fruct3 and At beta fruct4 and a gene encoding the apoplasmic invertase, At beta fruct1/AtcwINV1, are induced by gibberellin synthesized de novo following irradiation with red light. |
| Databáze: | OpenAIRE |
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