Pulsed Radiofrequency Neuromodulation Contributes to Activation of Platelet-Rich Plasma in In Vitro Conditions
| Autor: | Anna Michno, Ewelina Gojtowska, Zbigniew Kirkor, Bartosz Baścik, Irmina Śmietańska, Marek Suchorzewski |
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| Rok vydání: | 2021 |
| Předmět: |
Blood Platelets
Pharmacology 03 medical and health sciences 0302 clinical medicine Thrombin Neuromodulation Humans Medicine Platelet Platelet activation Wound Healing Platelet-Rich Plasma business.industry General Medicine Metabolism digestive system diseases In vitro Pulsed Radiofrequency Treatment Anesthesiology and Pain Medicine medicine.anatomical_structure Neurology Platelet-rich plasma Neurology (clinical) business 030217 neurology & neurosurgery Platelet factor 4 medicine.drug |
| Zdroj: | Neuromodulation: Technology at the Neural Interface. 24:1451-1457 |
| ISSN: | 1094-7159 |
| DOI: | 10.1111/ner.13105 |
| Popis: | Objectives Recent years have brought new developments in interventional chronic pain management, namely regenerative orthopedics utilizing platelet-rich plasma (PRP) as well as further evolution of pulsed radiofrequency neuromodulation (PRF). Both methods have been used separately. Here, we investigated whether PRF may potentiate the activation of platelets in PRP samples when both these techniques are combined together in in vitro conditions. Materials and methods Studies were performed on concentrated PRP samples (PRPs) obtained from acid citrate dextrose-treated blood taken from 11 healthy volunteers. PRPs were divided into four groups: 1) nonactivated PRP; 2) thrombin-activated PRP as a positive control for maximal platelets activation; 3) PRF-treated PRP exposed for 20 min to PRF energy generated by neurotherm radio frequency generator at 500 kHz, with a voltage of 40 V and maximal temperature of 42°C; and 4) a combination of groups 2 and 3. Results PRF-induced platelet activation measured by platelet factor 4 (PF4) and ATP release from PRPs was significantly higher compared to nonactivated PRPs, and similar to PF4 and ATP release from thrombin-activated PRPs. Thrombin activation did not potentiate PF4 release in PRF samples and even reduced ATP level. Additionally, PRF neither induced any platelet membrane damage measured by lactic dehydrogenase release from PRP nor modified any platelets viability or metabolism measured by MTT. Conclusions We confirmed that PRF may activate PRP without additional platelet activators. So, a combination of both methods PRF and PRP application may provide a more effective opportunity for tissue regeneration in dentistry, surgery, dermatology, or in orthopedics. |
| Databáze: | OpenAIRE |
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