LncRNA SNHG7 is an Oncogenic Biomarker Interacting with MicroRNA-193b in Colon Carcinogenesis
| Autor: | Lao Yq, Li Ns, Wu J, Li Wk, Liu Kl, Li Q |
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| Rok vydání: | 2019 |
| Předmět: |
Adenoma
MAPK/ERK pathway 030213 general clinical medicine Carcinogenesis Colon Apoptosis Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences HT29 Cells 0302 clinical medicine Downregulation and upregulation Sequence Homology Nucleic Acid microRNA Biomarkers Tumor medicine Humans Gene silencing 3' Untranslated Regions Cell Proliferation Base Sequence Cancer medicine.disease digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs HEK293 Cells Colonic Neoplasms Cancer research Biomarker (medicine) RNA Interference RNA Long Noncoding |
| Zdroj: | Clinical Laboratory. 65 |
| ISSN: | 1433-6510 |
| Popis: | BACKGROUND The expression and significance of small nucleolar RNA host gene 7 (SNHG7) in early-stage colon carcinogenesis remains unclear. METHODS The level of SNHG7 and microRNA-193b (miR-193b) was detected by qRT-PCR in colon tumor tissues and cells. The interaction of SNHG7 and miR-193b and the influence of SNHG7 silencing on colon tumor cells was evaluated. RESULTS Stepwise upregulated SNHG7 in colon advanced adenomas and early-stage cancer negatively correlates with miR-193b level, the direct interaction was confirmed in vitro. SNHG7 silencing in HT29 cells decreased proliferation and promoted apoptosis by inhibiting K-ras/ERK/cyclinD1. CONCLUSIONS SNHG7 is an oncogenic biomarker in colon carcinogenesis. The effect may be mediated by interaction with miR-193b. |
| Databáze: | OpenAIRE |
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