Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas

Autor: Caterina Longo, Maria Concetta Fargnoli, Claudia Martorelli, Gianluca Cipolloni, Elvira Moscarella, Ambra Antonini, Roberto Alfano, Giuseppe Argenziano, Alessia Ciarrocchi, Cristina Pellegrini, Marco Manfredini, Emi Dika, Ketty Peris, Riccardo Pampena
Přispěvatelé: Moscarella E., Pellegrini C., Pampena R., Argenziano G., Manfredini M., Martorelli C., Ciarrocchi A., Dika E., Peris K., Antonini A., Cipolloni G., Alfano R., Longo C., Fargnoli M.C., Moscarella, Elvira, Pellegrini, Cristina, Pampena, Riccardo, Argenziano, Giuseppe, Manfredini, Marco, Martorelli, Claudia, Ciarrocchi, Alessia, Dika, Emi, Peris, Ketty, Antonini, Ambra, Cipolloni, Gianluca, Alfano, Roberto, Longo, Caterina, Fargnoli, Maria Concetta
Rok vydání: 2019
Předmět:
0301 basic medicine
Oncology
Adult
Male
Proto-Oncogene Proteins B-raf
medicine.medical_specialty
Skin Neoplasms
molecular analyse
Adolescent
Concordance
DNA Mutational Analysis
Dermoscopy
Dermatology
Independent predictor
Biochemistry
BRAF
DNA Mutational Analysi
030207 dermatology & venereal diseases
03 medical and health sciences
Young Adult
0302 clinical medicine
Similarity (network science)
Retrospective Studie
molecular analyses
Internal medicine
medicine
Mutational status
Humans
Statistical analysis
Skin Neoplasm
neoplasms
Molecular Biology
Melanoma
Aged
Retrospective Studies
dermoscopy
multiple primary melanomas
multiple primary melanoma
business.industry
High-Throughput Nucleotide Sequencing
Middle Aged
030104 developmental biology
Tissue sections
Female
Settore MED/35 - MALATTIE CUTANEE E VENEREE
business
Human
Zdroj: Experimental dermatology. 28(7)
ISSN: 1600-0625
Popis: Background The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAFV600 mutational status was analysed with allele-specific TaqManTM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAFV600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.
Databáze: OpenAIRE
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