Cotranslational Integration of Soybean (Glycine max) Oil Body Membrane Protein Oleosin into Microsomal Membranes

Autor:	Eliot M. Herman, Deborah S. Loer
Rok vydání:	1993
Předmět:	Physiology Endoplasmic reticulum Plant Science Biology Membrane Oil body Biochemistry Membrane protein Organelle Glycine Genetics Microsome Oleosin Research Article
Zdroj:	Plant Physiology. 101:993-998
ISSN:	1532-2548 0032-0889
Popis:	Storage triglycerides in oil seeds are sequestered in discrete organelles termed oil bodies. They are bounded by a monolayer of phospholipids in which a few distinct proteins (oleosins) are embedded. Synthesis of soybean (Glycine max) 24-kD oleosin was analyzed by in vitro transcription and translation in reticulocyte lysate in the presence of canine microsomes. Our results show that 24-kD oleosin is cotranslationally integrated into microsomal membranes. We demonstrate that oleosin is integrated into a bilayer membrane in preference to the oil body monolayer membrane, indicating that oleosin is synthesized on the endoplasmic reticulum (ER). A new model of oil body assembly involving a conformational change through initial association with the ER membrane is proposed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd54eb0ff0d6918f7513c6bd112a20b8 https://doi.org/10.1104/pp.101.3.993 Zobrazit plný text záznamu