Evaluation of recombinant Kunjin replicon SIV vaccines for protective efficacy in macaques
| Autor: | Andreas Suhrbier, Erik Rollman, Vlad Mokhonov, Viv Peut, Xiang Ju Wang, Ekaterina I. Mokhonova, Alexander A. Khromykh, Sheilajen Alcantara, Caroline S. Fernandez, Kim Wilson, Liyen Loh, Rosemarie D. Mason, Robert De Rose, Stephen J. Kent, Jeanette C. Reece |
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| Rok vydání: | 2008 |
| Předmět: |
Kunjin
T-Lymphocytes animal diseases viruses Simian Acquired Immunodeficiency Syndrome Viremia Antibodies Viral Lymphocyte Activation Macaques 03 medical and health sciences Virology medicine Animals Cytotoxic T cell Replicon HIV vaccine 030304 developmental biology AIDS Vaccines Vaccines Vaccines Synthetic 0303 health sciences biology 030302 biochemistry & molecular biology SAIDS Vaccines HIV virus diseases Pigtail macaque biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Fusion Proteins gag-pol 3. Good health Flavivirus SIV Immunization Immunology HIV-1 biology.protein Simian Immunodeficiency Virus Macaca nemestrina Antibody Genetic Engineering West Nile virus |
| Zdroj: | Virology. 374:528-534 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2008.01.006 |
| Popis: | Persistent gag-specific T cell immunity would be a useful component of an effective HIV vaccine. The Flavivirus Kunjin replicon was previously engineered to persistently express HIV gag and was shown to induce protective responses in mice. We evaluated Kunjin replicon virus-like-particles expressing SIVgag–pol in pigtail macaques. Kunjin-specific antibodies were induced, but no SIV-specific T cell immunity were detected. Following SIVmac251 challenge, there was no difference in SIV viremia or retention of CD4 T cells between Kunjin–SIVgag–pol vaccine immunized animals and controls. An amnestic SIV gag-specific CD8 T cell response associated with control of viremia was observed in 1 of 6 immunized animals. Refinements of this vector system and optimization of the immunization doses, routes, and schedules are required prior to clinical trials. |
| Databáze: | OpenAIRE |
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