Determinants of fetal leptin synthesis, fat mass, and circulating leptin concentrations in well-nourished ewes in late pregnancy
| Autor: | Claire T. Roberts, Michael E. Symonds, B. S. J. Yuen, E M Marrocco, P. L. Stagg, Isabella Caroline McMillen, Beverly S. Muhlhausler, Helen Budge, J. K. Pearse, K. G. Kauter, James R. McFarlane |
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| Přispěvatelé: | McMillen, Isabella Caroline, Muhlhausler, Beverly, Roberts, Claire, Yuen, B, Marrocco, E, Budge, Helen, Symonds, M, McFarlane, J, Kauter, K, Stagg, P, Pearse, J |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Blood Glucose
Leptin Shoulder medicine.medical_specialty medicine.medical_treatment Adipose tissue Gestational Age Biology Kidney Ion Channels Adipose capsule of kidney Mitochondrial Proteins Fetus Endocrinology Pregnancy Internal medicine medicine Animals Insulin RNA Messenger Uncoupling Protein 1 Sheep Osmolar Concentration Membrane Proteins Gestational age Fetal Blood Lipid Metabolism Thermogenin Adipose Tissue embryonic structures Pregnancy Animal Gestation Animal Nutritional Physiological Phenomena Female Carrier Proteins |
| Popis: | We have investigated the factors regulating leptin synthesis, fat deposition, and circulating leptin concentrations in fetuses of well nourished ewes in late pregnancy. Vascular catheters were surgically inserted in 17 pregnant ewes and their fetuses at 103–120 d gestation (term 147 3 d). Ewes were fed a diet providing either 100% (control; n 9) or approximately 155% (well fed; n 8) of the maintenance energy requirements and fetal perirenal and interscapular fat depots were collected at 139 –141 d gestation. There was a significant relationship between the relative mass of fetal unilocular fat and fetal glucose (relative mass of unilocular fat, 1.14; fetal glucose, 0.16; r 0.50; P < 0.04; n 17), but not insulin, concentrations in the control and well-fed groups. In contrast to the controls, there was a positive relationship between the relative abundance of leptin mRNA and fetal insulin, but not glucose, concentrations in fetal perirenal adipose tissue in the well-fed group. A moderate increase in maternal nutrition also resulted in a strong reciprocal relationship between uncoupling protein 1 and leptin expression in fetal perirenal adipose tissue in late gestation (well-fed group: uncoupling protein 1 mRNA:18S rRNA,0.51; leptin mRNA:-actin mRNA, 1.53; r 0.80; P < 0.02; n 8). These studies provide evidence that fetal glucose and insulin differentially regulate fetal fat deposition and leptin mRNA expression within the fetal perirenal fat depot in the well nourished animal during late gestation. (Endocrinology 144: 4947– 4954, 2003) |
| Databáze: | OpenAIRE |
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