Evaluation of a PCR assay on overgrown individual fecal samples cultured for Mycobacterium avium subsp. paratuberculosis
| Autor: | Saray, Rangel, Juan C, Arango-Sabogal, Olivia, Labrecque, Julie, Paré, Julie-Hélène, Fairbrother, Sébastien, Buczinski, Jean-Philippe, Roy, Geneviève, Côté, Vincent, Wellemans, Gilles, Fecteau |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
General Veterinary 040301 veterinary sciences 030106 microbiology Cattle Diseases 04 agricultural and veterinary sciences Real-Time Polymerase Chain Reaction Sensitivity and Specificity Mycobacterium avium subsp. paratuberculosis 0403 veterinary science Feces 03 medical and health sciences Paratuberculosis Animals Cattle |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 29:912-915 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/1040638717724837 |
| Popis: | Microbial overgrowth can interfere with Mycobacterium avium subsp. paratuberculosis (MAP) growth and detection. We estimated the percentage of positive samples by PCR performed on the incubated media of individual fecal samples classified as non-interpretable (NI) by bacteriologic culture of liquid media. A total of 262 liquid cultures declared NI and 88 samples declared negative were included in the study. MAP DNA was detected in 7 NI samples (2.7%; 95% CI: 1.1–5.4%) and in 1 negative sample (1.1%; 95% CI: 0.3–6.2%). The PCR allowed the detection of MAP-positive samples that had been missed in the initial bacteriologic culture. However, the benefit of these few additional positive results must be weighed against the additional costs incurred. Using PCR to classify overgrown cultures optimizes the detection process and eliminates the NI outcome. |
| Databáze: | OpenAIRE |
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