Prognostic impact of changes in base excision repair machinery in sporadic colorectal cancer
| Autor: | Jenifer Saffi, Daniel de Barcellos Azambuja, Natalia Motta Leguisamo, Helena de Castro e Gloria, Antonio Nocchi Kalil, Ernani Luis Rhoden |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology Adult Male medicine.medical_specialty Colorectal cancer DNA polymerase beta Pathology and Forensic Medicine DNA Glycosylases 03 medical and health sciences chemistry.chemical_compound XRCC1 0302 clinical medicine Internal medicine Medicine Humans Promoter Regions Genetic Pathological Antineoplastic Agents Alkylating DNA Polymerase beta Aged Aged 80 and over business.industry Cell Biology Base excision repair Middle Aged medicine.disease Prognosis 030104 developmental biology Lymphatic system X-ray Repair Cross Complementing Protein 1 chemistry DNA glycosylase 030220 oncology & carcinogenesis Immunohistochemistry Female business Colorectal Neoplasms |
| Zdroj: | Pathology, research and practice. 214(1) |
| ISSN: | 1618-0631 |
| Popis: | Objective to evaluate the prognostic value of base excision repair proteins in sporadic colorectal cancer. Methods Pre-treatment tumor samples from 72 patients with sporadic colorectal adenocarcinoma were assessed for APC, MPG, Polβ, XRCC1 and Fen1 expression by immunohistochemistry. The associations of molecular data were analyzed in relation to clinical features and TNM staging as a prognosis predictor and disease-free survival. Results Higher levels of MPG, Polβ and XRCC1, but not Fen1, were associated with unfavorable pathological outcomes, such as poor cellular differentiation, advanced TNM stages, presence of lymphatic and perineural invasions and metastatic lymph nodes. MPG and Polβ overexpression were associated with right-sided CRC. However, only MPG high expression is associated with shorter disease-free survival in CRC patients. Conclusions Our results suggest that increased expression of MPG, Polβ and XRCC1 are more likely to evolve to poor pathological outcomes, but only the elevated expression of MPG protein predicts recurrence. The BER proteins appear to be suitable candidates to refine the TNM current staging of colorectal cancer. |
| Databáze: | OpenAIRE |
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