HIV Type 1 Subtype Surveillance in Central Kenya
| Autor: | Sheila Kageha, Samoel Khamadi, Raphael W. Lihana, Elijah M. Songok, Jane M. Ngaira, Fredrick A. Okoth, Vincent Okoth, Matilu Mwau |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Molecular Sequence Data Immunology Human immunodeficiency virus (HIV) Proviral dna Biology medicine.disease_cause Gp41 Polymerase Chain Reaction Peripheral blood mononuclear cell Virus law.invention law Virology HIV Seropositivity medicine Humans Phylogeny Polymerase chain reaction Genetic diversity Direct sequencing Genetic Variation Sequence Analysis DNA Kenya Cross-Sectional Studies Infectious Diseases DNA Viral HIV-1 Female |
| Zdroj: | AIDS Research and Human Retroviruses. 28:228-231 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | Human immunodeficiency virus 1 (HIV-1) infection is characterized by genetic diversity such that specific viral subtypes are predominant in specific geographic areas. To determine circulating subtypes of HIV-1 in different parts of central Kenya, a cross-sectional study was carried out on HIV-1-positive blood samples collected from consenting individuals in eight hospitals of Kenya's central province. Proviral DNA was extracted from peripheral blood mononuclear cells. Polymerase chain reaction and direct sequencing using primers generated from a highly conserved region of HIV-1 env gp41 were carried out. Ninety-six samples were successfully amplified and sequenced. Analysis of the sequences showed that a majority of them belonged to subtype A1 (67/96, 69.8%), followed by subtypes D (18, 18.7%) and C (11/96, 11.5%). Consistent with findings in other parts of Kenya, HIV-1 subtype A1 was the most dominant virus in circulation. Continued surveillance of circulating subtypes of HIV-1 in Kenya is important in determining the evolution of the HIV/AIDS epidemic in Kenya. |
| Databáze: | OpenAIRE |
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