Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells

Autor: Kyung Dae Ko, Jordan Krebs, Vittorio Sartorelli, Xuesong Feng, Roger A. Pedersen, Rafael Casellas, Hossein Zare, Adam L. Knight, Karinna O. Vivanco, Aster H. Juan, Pei Fang Tsai, Gustavo Gutierrez-Cruz, Anthony M. Ascoli, Benjamin A. Garcia, Simone Sidoli, Jizhong Zou, Stan Wang
Přispěvatelé: Massachusetts Institute of Technology. Department of Biological Engineering, Vivanco, Karinna O.
Jazyk: angličtina
Předmět:
0301 basic medicine
Transcription
Genetic
Cellular differentiation
Cell
H3K27 methylation
Embryoid body
macromolecular substances
Regulatory Sequences
Nucleic Acid
Methylation
General Biochemistry
Genetics and Molecular Biology
Article
Histones
Mice
03 medical and health sciences
Histone H3
Transcription Activator-Like Effector Nucleases
medicine
Animals
Cell Lineage
Enhancer of Zeste Homolog 2 Protein
Psychological repression
lcsh:QH301-705.5
Embryoid Bodies
Neurons
Regulation of gene expression
Genetics
Genome
biology
Lysine
EZH2
fungi
Cell Differentiation
Mouse Embryonic Stem Cells
embryonic stem cells
Embryonic stem cell
Cell biology
3. Good health
medicine.anatomical_structure
030104 developmental biology
polycomb proteins
Gene Expression Regulation
lcsh:Biology (General)
biology.protein
RNA Editing
PRC2
Zdroj: Elsevier
Cell Reports, Vol 17, Iss 5, Pp 1369-1382 (2016)
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2016.09.087
Popis: The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.
National Institute of Arthritis and Musculoskeletal and Skin Diseases (U.S.). Intramural Research Program
Databáze: OpenAIRE
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