Osmoregulatory role of vasotocinergic and isotocinergic systems in the gilthead sea bream (Sparus aurata L)
| Autor: | Juan Antonio Martos-Sitcha, Gonzalo Martínez-Rodríguez, Juan Miguel Mancera, Arleta K. Skrzynska |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Receptors Vasopressin Biology Stress Acclimatization 03 medical and health sciences Endocrinology Vasotocin Osmoregulation Aquaculture Sparus aurata Internal medicine Gene expression medicine Animals Receptor Arginine Vasotocin business.industry Isotocin Metabolism Sea Bream Salinity 030104 developmental biology Animal Science and Zoology business Arginine vasotocin |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 8th International Symposium on Fish Endocrinology (8ISFE), celebrado en Gotemburgo del 28 de junio al 2 de julio de 2016. Gilthead sea bream, Sparus aurata L., is an importan\ fish species for the Mediterranean aquaculture and is considered a good model for the study of osmoregulatory processes due to its capacity to cope with great changes in environmental salinity (5-60 ppt). Our group studied the osmoregulatory role of different endocrine systems in this species, focussing on the vasotocinergic and isotocinergic systems during the last years. For this purpose, cDNAs for pro-AVT, pro-IT, two AVT receptors (V1a2- and V2-types) and one IT receptor (ITR) were cloned. Acclimation to different environmental salinities induced a direct lineal relationship between plasma AVT levels and salinity. w'ith no changes in plasma IT values. In addition, higher values in hypothalamic pro-VT and pro-IT mRNA expression, correlated with changes in plasma cortisol levels, as well as with pituitary AVT and IT storage levels in both hypo- and/or hyper-osmotic transfers, suggesting an interaction between cortisol and AVT/IT pathways. Moreover, mRNA expression of specific receptors demonstrated an importan\ osmoregulatory orchestration in different organs. The use of different in vitro techniques indicated: i) a modulatory role of AVT and IT in Cl- secretion across the opercular epithelium mediated by different ion transporters: ii) a sflmulatory role of A VT in the regulation of intestinal ion absorption: and iii) a functional involvement of AVT and IT in the stimulation of intestinal water transport via AOP1 paralogs. promoting acclimation to high salinity environments. In addition, specimens intraperitoneally injected with AVT and transferred to LSW or HSW enhanced plasma cortisol levels and/or gill Na",K.-ATPase activity. These effects could be related to the energy repartitioning process occurring during osmotic adaptation of S. aurata to extreme environmental salinities. which could be mediated not only by plasma cortisol but also by AVT. Finally, our results indicated a very importan\ role of the vasotocinergic and/or isotocinergic systems in both osmoregulatory and non-osmoregulatory organs. The work has been carried out under Campus de Excelencia International del Mar and under the project AGL2013-48835-C2-R (Ministerio de Economía y Competitividad, Spain) |
| Databáze: | OpenAIRE |
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