Alternatives for obtaining a continuous cell line from Apis mellifera
| Autor: | Silvia de Oliveira Hübner, Gilberto D'Ávila Vargas, Lariane da Silva Barcelos, Marcelo de Lima, Geferson Fischer, Matheus Iuri Frühauf, Nadálin Yandra Botton, Cristina Mendes Peter |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Agriculture (General) Computational biology Biology honeybee immortalization S1-972 03 medical and health sciences 0302 clinical medicine Genotype cell culture Cell immortalization General Veterinary food and beverages Agriculture Honey bee cell line Phenotype 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Continuous cell line Animal Science and Zoology Agronomy and Crop Science Immortalised cell line In vitro cell culture |
| Zdroj: | Ciência Rural v.51 n.12 2021 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM Ciência Rural, Vol 51, Iss 12 (2021) |
| Popis: | In worldwide there are reports of a significant decrease in colonies of the species Apis mellifera, caused by several factors, including viral infections. In order to study and diagnose illnesses caused by viruses, in vitro cell culture is used as a valuable tool. Yet, there are still no immortalized cell lines of honey bee Apis mellifera. Primary cell cultures are promising for this purpose and can supply the lack of continuous strains, but their establishment is difficult and laborious, which often makes them unfeasible for many research centers. Through the use of cell immortalization techniques, it is possible to develop continuous cell lines and thus benefit, in different ways, research related to different species of bees. The choice of technique is challenging, since in addition to the ability to remain viable for countless passages, cells must keep the genotype and phenotype similar or identical to the original tissue. This review intends to present methodologies that can be used to immortalize Apis mellifera cells, aiming to establish a cell line. The genotypic and phenotypic implications of each technique are evaluated, and the purpose of the cell line to be developed. |
| Databáze: | OpenAIRE |
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