Genome-Wide Gene Expression Analysis Suggests an Important Role of Suppressed Immunity in Pathogenesis of Kashin-Beck Disease
Autor: | Shuang Wang, Mikko J. Lammi, Xiaoming Wu, Xiong Guo |
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Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Male
Microarrays Gene Identification and Analysis lcsh:Medicine Gene Expression Gene expression lcsh:Science Immune Response Oligonucleotide Array Sequence Analysis Kashin-Beck Disease Multidisciplinary Genomics Middle Aged Reverse transcription polymerase chain reaction Medicine Female DNA microarray Research Article Adult Immunology Biology Peripheral blood mononuclear cell Immune Suppression Molecular Genetics Rheumatology Genome Analysis Tools Osteoarthritis medicine Genome-Wide Association Studies Immune Tolerance Humans Gene Regulation Gene Aged Kashin–Beck disease Microarray analysis techniques Genome Human Gene Expression Profiling lcsh:R Immunity Computational Biology Immunoregulation Reproducibility of Results medicine.disease Gene expression profiling Cartilage Case-Control Studies lcsh:Q |
Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 1, p e28439 (2012) |
ISSN: | 1932-6203 |
Popis: | Objective To investigate the differences between the gene expression profiles in peripheral blood mononuclear cells (PBMC) from normal controls and patients with Kashin-Beck disease (KBD). Methods Twenty KBD patients and 12 normal subjects were selected from a KBD-endemic area and divided into four pairs of KBD vs. control (KBD, n = 5 per pair; control, n = 3 per pair). RNAs were respectively isolated from KBD PBMCs and normal PBMCs. Gene expression profiles were analyzed by oligonucleotide microarray. The gene expression profiles in PBMCs from KBD patients and normal controls were compared and the differentially expressed genes were identified. The obtained microarray data was further confirmed by using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Results Approximately 501 genes, corresponding to 2.4% of the total probe transcripts, showed a 2-fold change in differential expression. 19.4% (97 out of 501)of the differentially expressed genes were commonly detected in all the four pairs. Among the 97 differentially expressed genes, 83 genes were up-regulated and 14 genes were down-regulated, compared with those in the normal controls. Some differentially expressed genes were found to be related to functions such as immunity, metabolism, apoptosis, cystoskeleton and cell movement, and extracellular matrix. The validity of our microarray data were supported by the results of qRT-PCR assay. Conclusion Differences in the PBMC gene expression profile between the KBD patients and the normal controls exhibited a similar pattern among all the four pairs of microarrays examined, indicating that the suppressed immunity may play an important role in the pathogenesis of KBD. |
Databáze: | OpenAIRE |
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