Genome-Wide Gene Expression Analysis Suggests an Important Role of Suppressed Immunity in Pathogenesis of Kashin-Beck Disease

Autor: Shuang Wang, Mikko J. Lammi, Xiaoming Wu, Xiong Guo
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Microarrays
Gene Identification and Analysis
lcsh:Medicine
Gene Expression
Gene expression
lcsh:Science
Immune Response
Oligonucleotide Array Sequence Analysis
Kashin-Beck Disease
Multidisciplinary
Genomics
Middle Aged
Reverse transcription polymerase chain reaction
Medicine
Female
DNA microarray
Research Article
Adult
Immunology
Biology
Peripheral blood mononuclear cell
Immune Suppression
Molecular Genetics
Rheumatology
Genome Analysis Tools
Osteoarthritis
medicine
Genome-Wide Association Studies
Immune Tolerance
Humans
Gene Regulation
Gene
Aged
Kashin–Beck disease
Microarray analysis techniques
Genome
Human
Gene Expression Profiling
lcsh:R
Immunity
Computational Biology
Immunoregulation
Reproducibility of Results
medicine.disease
Gene expression profiling
Cartilage
Case-Control Studies
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 1, p e28439 (2012)
ISSN: 1932-6203
Popis: Objective To investigate the differences between the gene expression profiles in peripheral blood mononuclear cells (PBMC) from normal controls and patients with Kashin-Beck disease (KBD). Methods Twenty KBD patients and 12 normal subjects were selected from a KBD-endemic area and divided into four pairs of KBD vs. control (KBD, n = 5 per pair; control, n = 3 per pair). RNAs were respectively isolated from KBD PBMCs and normal PBMCs. Gene expression profiles were analyzed by oligonucleotide microarray. The gene expression profiles in PBMCs from KBD patients and normal controls were compared and the differentially expressed genes were identified. The obtained microarray data was further confirmed by using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Results Approximately 501 genes, corresponding to 2.4% of the total probe transcripts, showed a 2-fold change in differential expression. 19.4% (97 out of 501)of the differentially expressed genes were commonly detected in all the four pairs. Among the 97 differentially expressed genes, 83 genes were up-regulated and 14 genes were down-regulated, compared with those in the normal controls. Some differentially expressed genes were found to be related to functions such as immunity, metabolism, apoptosis, cystoskeleton and cell movement, and extracellular matrix. The validity of our microarray data were supported by the results of qRT-PCR assay. Conclusion Differences in the PBMC gene expression profile between the KBD patients and the normal controls exhibited a similar pattern among all the four pairs of microarrays examined, indicating that the suppressed immunity may play an important role in the pathogenesis of KBD.
Databáze: OpenAIRE
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