Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids
| Autor: | Chih Chien Hsu, Shih Jie Chou, Shih Hwa Chiou, Tai Chi Lin, Yi Ping Yang, Yueh Chien, Chian Shiu Chien, De Kuang Hwang, Ying Chun Jheng, Henkie Isahwan Ahmad Mulyadi Lai, Ping Hsing Tsai, Yu Bai Chou, Shih-Jen Chen, Mong Lien Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
induced pluripotent stem cells Cell Culture Techniques Gene Expression ACE2 Biology spike protein TMPRSS2 Catalysis Retina Article Green fluorescent protein Cell Line Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Organoid medicine Humans Physical and Theoretical Chemistry Receptor Induced pluripotent stem cell Molecular Biology lcsh:QH301-705.5 Spectroscopy SARS-CoV-2 Organic Chemistry fungi Serine Endopeptidases COVID-19 SARS-CoV-2 pseudovirus Retinal General Medicine Virus Internalization Computer Science Applications Cell biology Organoids 030104 developmental biology medicine.anatomical_structure chemistry lcsh:Biology (General) lcsh:QD1-999 Cell culture Angiotensin-Converting Enzyme 2 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 1320, p 1320 (2021) Volume 22 Issue 3 |
| ISSN: | 1422-0067 |
| Popis: | Angiotensin-converting enzyme 2 (ACE2) was identified as the main host cell receptor for the entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its subsequent infection. In some coronavirus disease 2019 (COVID-19) patients, it has been reported that the nervous tissues and the eyes were also affected. However, evidence supporting that the retina is a target tissue for SARS-CoV-2 infection is still lacking. This present study aimed to investigate whether ACE2 expression plays a role in human retinal neurons during SARS-CoV-2 infection. Human induced pluripotent stem cell (hiPSC)-derived retinal organoids and monolayer cultures derived from dissociated retinal organoids were generated. To validate the potential entry of SARS-CoV-2 infection in the retina, we showed that hiPSC-derived retinal organoids and monolayer cultures endogenously express ACE2 and transmembrane serine protease 2 (TMPRSS2) on the mRNA level. Immunofluorescence staining confirmed the protein expression of ACE2 and TMPRSS2 in retinal organoids and monolayer cultures. Furthermore, using the SARS-CoV-2 pseudovirus spike protein with GFP expression system, we found that retinal organoids and monolayer cultures can potentially be infected by the SARS-CoV-2 pseudovirus. Collectively, our findings highlighted the potential of iPSC-derived retinal organoids as the models for ACE2 receptor-based SARS-CoV-2 infection. |
| Databáze: | OpenAIRE |
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