Ductular reactions in the liver regeneration process with local inflammation after physical partial hepatectomy
| Autor: | Kohei Kume, Yasuhiro Takikawa, Hirokatsu Katagiri, Satoshi Nishizuka, Yuji Suzuki, Ting Wang, Keisuke Kakisaka |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty medicine.medical_treatment Inflammation Pathology and Forensic Medicine Extracellular matrix 03 medical and health sciences Hepatic Stellate Cells medicine Extracellular Animals Hepatectomy Progenitor cell Molecular Biology Cell Proliferation Tumor Necrosis Factor-alpha Chemistry Gene Expression Profiling Stem Cells Regeneration (biology) NF-kappa B Hypertrophy Cell Biology Actins Matrix Metalloproteinases Liver regeneration Extracellular Matrix I-kappa B Kinase Liver Regeneration Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Liver Anesthesia Hepatocyte Models Animal Hepatocytes Female medicine.symptom |
| Zdroj: | Laboratory Investigation. 96:1211-1222 |
| ISSN: | 0023-6837 |
| Popis: | Partial hepatectomy models in mice have been widely used for liver regeneration studies. A typical procedure removes ~2/3 of the liver by lobular ligation without tissue dissection. However, hepatectomy in humans involves physical damage (ie, physical partial hepatectomy, PPHx). Therefore, the liver regeneration process after PPHx should involve reactions to acute local injury followed by systematic remodeling. To clarify the liver regeneration process after PPHx, we used a murine liver injury model that mimics the actual human surgical procedure. A 20-30% PPHx was performed by transection of the left lobe of the liver using an ultrasonically activated scalpel in mice. Gene expression and morphological characteristics were analyzed during the liver regeneration process. Liver weight continuously increased by hypertrophic reaction of hepatocytes, whereas Ki67 staining showed hepatocyte proliferation. At the transected border, emergence of ductular reactions, a representative process of hepatic tissue remodeling that contain liver stem/progenitor cells, were observed. Gene expression of the transected border and non-damaged lobes revealed that inflammatory cytokine- and extracellular matrix-associated genes were significantly upregulated at the transected border. Our PPHx model triggered local extracellular matrix remodeling that resulted in ductular reactions. These processes occurred during the tissue repair process in local inflammatory responses as well as compensatory hepatocyte hypertrophy of the entire liver. These findings may provide insight for elucidating the mechanism of tissue repair and regeneration of the liver after PPHx. |
| Databáze: | OpenAIRE |
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