The Establishment of an In Vivo HIV-1 Infection Model in Humanized B-NSG Mice
| Autor: | Li Sun, Wei-Wei Sun, Xian-Guang Yang, Tian-Jiao Fan, Jian-Hua Wang, Xia Jin |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Viral pathogenesis T cell 030106 microbiology Immunology HIV Infections Mice Transgenic Biology Virus Replication Peripheral blood mononuclear cell Virus 03 medical and health sciences Mice In vivo Mice Inbred NOD Virology medicine Animals Humans T lymphocyte Viral Load Phenotype Specific Pathogen-Free Organisms Disease Models Animal 030104 developmental biology medicine.anatomical_structure Viral replication HIV-1 Leukocytes Mononuclear Molecular Medicine Female Research Article |
| Zdroj: | Virol Sin |
| ISSN: | 1995-820X |
| Popis: | Suitable animal models for human immunodeficiency virus type 1 (HIV-1) infection are important for elucidating viral pathogenesis and evaluating antiviral strategies in vivo. The B-NSG (NOD-PrkdcscidIl2rgtm1/Bcge) mice that have severe immune defect phenotype are examined for the suitability of such a model in this study. Human peripheral blood mononuclear cells (PBMCs) were engrafted into B-NSG mice via mouse tail vein injection, and the repopulated human T-lymphocytes were observed at as early as 3-weeks post-transplantation in mouse peripheral blood and several tissues. The humanized mice could be infected by HIV-1, and the infection recapitulated features of T-lymphocyte dynamic observed in HIV-1 infected humans, meanwhile the administration of combination antiretroviral therapy (cART) suppressed viral replication and restored T lymphocyte abnormalities. The establishment of HIV-1 infected humanized B-NSG mice not only provides a model to study virus and T cell interplays, but also can be a useful tool to evaluate antiviral strategies. |
| Databáze: | OpenAIRE |
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