Mechanistic insights into plant SUVH family H3K9 methyltransferases and their binding to context-biased non-CG DNA methylation
| Autor: | Zhenhui Zhong, Jiamu Du, Rui Liu, C.J. Harris, Zonghua Wang, Baoqian Jia, Steven E. Jacobsen, Sisi Li, Xuezhen Li, Wei Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Models Molecular Methyltransferase Protein Conformation 1.1 Normal biological development and functioning Arabidopsis Genetically Modified plant Crystallography X-Ray Methylation H3K9me2 Histones 03 medical and health sciences chemistry.chemical_compound Models Underpinning research Genetics SUVH6 Regulation of gene expression Cofactor binding Multidisciplinary Crystallography DNA methylation biology Base Sequence Chemistry Arabidopsis Proteins histone methyltransferase Lysine Human Genome Molecular DNA Histone-Lysine N-Methyltransferase Plants DNA Methylation Plants Genetically Modified 030104 developmental biology Histone PNAS Plus Histone methyltransferase biology.protein X-Ray Nucleic Acid Conformation Generic health relevance Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 115, iss 37 |
| Popis: | DNA methylation functions in gene silencing and the maintenance of genome integrity. In plants, non-CG DNA methylation is linked through a self-reinforcing loop with histone 3 lysine 9 dimethylation (H3K9me2). The plant-specific SUPPRESSOR OF VARIEGATION 3–9 HOMOLOG (SUVH) family H3K9 methyltransferases (MTases) bind to DNA methylation marks and catalyze H3K9 methylation. Here, we analyzed the structure and function of Arabidopsis thaliana SUVH6 to understand how this class of enzyme maintains methylation patterns in the genome. We reveal that SUVH6 has a distinct 5-methyl-dC (5mC) base-flipping mechanism involving a thumb loop element. Autoinhibition of H3 substrate entry is regulated by a SET domain loop, and a conformational transition in the post-SET domain upon cofactor binding may control catalysis. In vitro DNA binding and in vivo ChIP-seq data reveal that the different SUVH family H3K9 MTases have distinct DNA binding preferences, targeting H3K9 methylation to sites with different methylated DNA sequences, explaining the context biased non-CG DNA methylation in plants. |
| Databáze: | OpenAIRE |
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