Behavioral and brain- transcriptomic synchronization between the two opponents of a fighting pair of the fish Betta splendens

Autor: Hao Ven Wang, Kenshiro Oshima, Y. W. Lin, Takashi Gojobori, H. Sunny Sun, Akiko Maruko, Satoshi Tamaki, Wen-Hsiung Li, Chih-Kuan Chen, Shun Fen Tzeng, Takashi Abe, Mei Yeh Lu, Erica Iioka, Yuki Iwasaki, Masaru Hojo, Shuji Shigenobu, Chao Li Huang, Hao Jen Huang, Norihiro Okada, Tzen Yuh Chiang, Akio Kanai, Trieu Duc Vu
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Cell signaling
Cancer Research
Video Recording
Gene Expression
Social Sciences
Signal transduction
QH426-470
Transcriptome
Sequencing techniques
Learning and Memory
0302 clinical medicine
Synchronization (computer science)
Psychology
RNA-Seq
Cooperative Behavior
Mating
Zebrafish
Genetics (clinical)
0303 health sciences
Behavior
Animal
Animal Behavior
Fishes
Brain
Eukaryota
Signaling cascades
RNA sequencing
Genomics
Animal Models
Aggression
Experimental Organism Systems
Osteichthyes
Vertebrates
Fish
medicine.symptom
Betta splendens
Behavior Observation Techniques
Transcriptome Analysis
Research Article
Cell biology
MAPK signaling cascades
Fish Biology
Biology
03 medical and health sciences
Model Organisms
Memory
Fish Physiology
medicine
Genetics
Animals
Learning
Animal Physiology
Interpersonal Relations
Molecular Biology
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Behavior
Ion Transport
Organisms
Biology and Life Sciences
Computational Biology
Behavioral pattern
Genome Analysis
biology.organism_classification
Vertebrate Physiology
Research and analysis methods
Molecular biology techniques
Fish
Biting
Gene Expression Regulation
Evolutionary biology
Animal Studies
Zoology
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS Genetics, Vol 16, Iss 6, p e1008831 (2020)
PLoS Genetics
ISSN: 1553-7404
1553-7390
Popis: Conspecific male animals fight for resources such as food and mating opportunities but typically stop fighting after assessing their relative fighting abilities to avoid serious injuries. Physiologically, how the fighting behavior is controlled remains unknown. Using the fighting fish Betta splendens, we studied behavioral and brain-transcriptomic changes during the fight between the two opponents. At the behavioral level, surface-breathing, and biting/striking occurred only during intervals between mouth-locking. Eventually, the behaviors of the two opponents became synchronized, with each pair showing a unique behavioral pattern. At the physiological level, we examined the expression patterns of 23,306 brain transcripts using RNA-sequencing data from brains of fighting pairs after a 20-min (D20) and a 60-min (D60) fight. The two opponents in each D60 fighting pair showed a strong gene expression correlation, whereas those in D20 fighting pairs showed a weak correlation. Moreover, each fighting pair in the D60 group showed pair-specific gene expression patterns in a grade of membership analysis (GoM) and were grouped as a pair in the heatmap clustering. The observed pair-specific individualization in brain-transcriptomic synchronization (PIBS) suggested that this synchronization provides a physiological basis for the behavioral synchronization. An analysis using the synchronized genes in fighting pairs of the D60 group found genes enriched for ion transport, synaptic function, and learning and memory. Brain-transcriptomic synchronization could be a general phenomenon and may provide a new cornerstone with which to investigate coordinating and sustaining social interactions between two interacting partners of vertebrates.
Author summary Agonistic encounters induce changes in the brain and behavior, but their underlying molecular mechanisms remain poorly understood. The fighting fish Betta splendens are small freshwater fish that are well known for their aggressiveness and are widely used to study aggression. Here, by measuring aggressive behavior displays (bite/strike/surface-breathing) between two opponents during fighting, we demonstrate that the two opponents in each fighting pair showed similar fighting configurations by influencing each other. In addition, we compared brain gene expression between opponents and showed synchronization of gene expression within a fighting pair, leading to pair-specific synchronization in genes associated with ion transport, synapse function, and learning and memory. This study presents the possibility that similar behaviors in pairs of animals under similar conditions may trigger synchronizing waves of transcription between the individuals, providing a hint to support the idea that fighting behaviors contain cooperative aspects at the molecular level.
Databáze: OpenAIRE
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